Chapter 2&4 Flashcards
You can see what they look like, what they’re doing, and how they’re interacting.
Macroscopic
Introduces organisms.
Inoculation
That provides them with the nutrients and resources they need to grow.
Media
Removes all living things from an environment
Sterile
Most environments contain a variety of microbes, but scientist want to study one at a time.
Isolated
Is the process of storing the inoculation medium in a controlled environment to allow it to grow.
Incubation
This is done most easily on solid media, where you see individual colonies
pure culture
Is assumed to have come from one cell that has multiplied for many generations to create millions of identical cells.
Colony
You can’t know whether results are caused by organism you care about.
Contamination
Involves observing characteristics of organisms that you’ve grown
Inspection
Is the use of information gathered from inspection to determine what the organism actually is.
Identification
Will allow many different microbes to grow.
General purpose media
If its chemical composition is know completely it is considered.
Defined
If there is no way to know its exact chemical composition then its
Complex
Are media have had ‘special’ substance added
Enriched media
ones that have special ‘dietary requirements’
Fastidious
Media with ingredients meant to prevent the growth of certain organisms is considered
Selective
Media with ingredients that cause some organisms to show a visibly different growth is considered
Differential
This is the study of organisms too small to be seen by the naked eye and we use something to see them.
Microscopy
Works by passing light through a specimen, then through one or more lenses
Light microscopy
Is how much bigger an image looks compared to its actual size
Magnification
Is how far two things have to be apart for them to be distinguished under a microscope.
Resolution
Techniques to color and highlight cells and cellular features so we can see them under a microscope.
Staining
Dye that directly color the surface of the cell are known as
Direct (or positive) stains
Dye that color the slide itself but do not color the surface of the cell are known as
Indirect (or negative) stains
If a stain has more than one way of reacting with a cell it is known as
Differential stain
Are used to differentiate between two types of structure on the outside of the cell
Gram stains