Chapter 15 - Intracellular Compartments And Transport Flashcards
Invagination
Bending inward
What members does invagination
ER, golgi, endosomes, lysosomes
What are the two proposed mechanism of evolution of membrane enclosed organelles
Membrane invagination
Endosymbiosis
Nuclear membranes and the ER are believed to have evolved from invagination from where
Plasma membrane
Mitochondria and chloroplast have through
Endosymbiosis
Mitochondria and chloroplast replicate by
Binary fission
Virtually all proteins are synthesized by ________ in the _____, then transported to their final destination
Ribosomes, cytosol
Sorting signal (signal sequence)
Directs the protein to the organelle in which it is required
Proteins destined to the ER posses what
N terminal signal sequence that directs them to ER
What happens if no signal sequence what happens to proteins
They remain in the cytosol
What are three different mechanisms of protein import into the organelles
Transport through nuclear pores
Transport across membranes
Transport vesicles
How does molecules move in and out of nucleus
Nuclear pores
What moves out of the nuclear pores
MRNAs and ribosomal subunits move out
What moves in nuclear protein, and what is needed for this to happen
Nuclear protein
A signal sequence
What can you say about water and nuclear pores
Water moves freely
What is needed to transport into the nucleus
Nuclear localization signal
Nuclear import receptor
Nuclear localization signal
Directs proteins from the cytosol into the nucleus
Nuclear import receptor
Directs the new protein through the nuclear pore
This is recycled and used for later use
What drives nuclear transport
Energy supplied by GTP hydrolysis
Ran GTP
GTP binding protein
What is unique about proteins passing through nuclear pores
They don’t need to unfold to pass through
Ran GTP
Is active
Ran GDP
Is inactive
Ran GAP
Triggers conversion of ran GTP to Ran GDP
Signal sequence of proteins targeted to the mitochondria and chloroplast binds to what
Receptor in the outer membrane
Complex of the receptor, protein, and protein translocator diffuses ________ in the outmembrane until it encounters _____ _____ in the _________
Laterally
Second protein translocator
Inner membrane
What happens when’s two protein translocator transport the protein across both membranes
The protein unfolds in the process
How does chaperons help with transporting proteins into the mitochondria and chloroplast
They are inside the organelle help pull the protein through and then refold it
Once a protein is transported in the mitochondria and chloroplast, what happens to the signal sequence
Cleaved off by signal peptidase
Proteins with multiple destinations first enter where
The ER
Examples with proteins with multiple destinations
ER proteins, Golgi proteins, endosomes proteins, lysosome proteins, and cell surface proteins
Once proteins enter the ER, they are ALWAYS what
Contained in the membrane enclosed organelle or compartment (endomembrane system)
What are the two types of proteins that enter the ER
Water soluble proteins
Transmembrane proteins
Water soluble proteins pass through what? And transport to where?
Pass completely through the ER membrane into the lumen, then transported into lumen of specific organelle or secreted to extracellular space
Transmembrane proteins transported though and transported where
Are PARTYLY transported through ER membrane and embedded to keep from transporting into lumen
Another name for cytosolic ribosomes
Free ribosomes
Cytosolic ribosomes
Ribosomes that are fee in the cytosol
Free ribosomes make what type of proteins and the proteins are destined where
Cytosolic proteins, proteins destined to the mitochondrial, nucleus, chloroplast and peroxisomes
ER bound ribosomes
Attached to the cytosolic side of the ER
Polyribosome
Many ribosomes bind to each mRNA
ER Signal sequence
On the polypeptide directs the ribosome translating the polypeptide to bind the cytosolic side of the ER
Polyribosomes are beneficial because
Allows a large number of polypeptides to be made from a single mRNA within a short period of time
What components help ribosome bind to the ER membrane
Signal recognition particle
SRP receptor
SRP or signal recognition protein
Binds to the ER signal sequence and ribosome
Slowing down polypeptide synthesis
SRP receptor
Binds to the SRP complex
Polypeptide passes through protein translocator into the ER lumen, speeding up polypeptide synthesis
Protein translocator opens up upon binding to what
Signal sequence
Where are water soluble proteins found? N and C termini
ER Lumen, The termini is found in the lumen too
Single pass transmembrane protein is kept in the ER by
Stop transfer sequence