CHAPTER 10 Flashcards
- The megakaryocyte progenitor that undergoes endomitosis is:
a. MK-I
b. BFU-Meg
c. CFU-Meg
d. LD-CFU-Meg
d. LD-CFU-Meg
- The growth factor that is produced in the kidney and induces growth and differentiation of committed
megakaryocyte progenitors is:
a. IL-3
b. IL-6
c. IL-11
d. Thrombopoietin
d. Thrombopoietin
- What platelet organelle sequesters ionic calcium and binds a series of enzymes of the eicosanoid pathway?
a. Glycocalyx
b. Dense granules
c. Dense tubular system
d. Surface connected canalicular system
c. Dense tubular system
- What platelet membrane receptor binds fibrinogen and supports platelet aggregation?
a. GP Ib/IX/V
b. GP IIb/IIIa
c. GP Ia/IIa
d. P2Y1
b. GP IIb/IIIa
- What platelet membrane phospholipid flips from the inner surface to the plasma surface on activation and serves as the assembly point for coagulation
factors?
a. Phosphatidylethanolamine
b. Phosphatidylinositol
c. Phosphatidylcholine
d. Phosphatidylserine
d. Phosphatidylserine
- What is the name of the eicosanoid metabolite produced from endothelial cells that suppresses platelet activity?
a. Thromboxane A2
b. Arachidonic acid
c. Cyclooxygenase
d. Prostacyclin
d. Prostacyclin
- Which of the following molecules is stored in platelet dense granules?
a. Serotonin
b. Fibrinogen
c. Platelet factor 4
d. Platelet-derived growth factor
a. Serotonin
- What plasma protein is essential for platelet adhesion?
a. VWF
b. Factor VIII
c. Fibrinogen
d. P-selectin
a. VWF
- Reticulated platelets can be enumerated in peripheral blood to detect:
a. Impaired platelet production in disease
states
b. Abnormal organelles associated with
diseases such as leukemia
c. Increased platelet production in
response to need
d. Inadequate rates of membrane
cholesterol exchange with the plasma
c. Increased platelet production in
response to need
- White clots:
a. Occur primarily in the deep veins of the
leg
b. Are characteristic of the secondary
hemostatic process
c. Are largely composed of platelets and
von Willebrand factor
d. Form normally in response to vascular
injury and are completely harmless
c. Are largely composed of platelets and
von Willebrand factor
- Upon activation, platelets secrete their a-granule contents via:
a. The dense tubule system
b. The surface connected canalicular
system
c. The glycocalyx
d. Microtubules
b. The surface connected canalicular
system
- Microparticles:
a. Are stored in platelet dense granules
b. Inhibit blood clotting
c. Bud off of platelets after their exposure
to strong agonists
d. Exhibit no biologic activity
c. Bud off of platelets after their exposure
to strong agonists
- A 1:20 dilution of blood is made with 3% glacial acetic acid as the diluent. The four large corner squares on both sides of the hemacytometer are counted, for a total of 100 cells. What is the total WBC count (3109 /L)?
a. 0.25
b. 2.5
c. 5
d. 10
b. 2.5
- The total WBC count is 20 3 109 /L. Twenty-five NRBCs per 100 WBCs are observed on the peripheral blood film. What is the corrected WBC count (3109 /L)?
a. 0.8
b. 8
c. 16
d. 19
c. 16
- If potassium cyanide and potassium ferricyanide are used in the manual method for hemoglobin determination, the final product is:
a. Methemoglobin
b. Azide methemoglobin
c. Cyanmethemoglobin
d. Myoglobin
c. Cyanmethemoglobin
- Which of the following would NOT interfere with the result when hemoglobin determination is performed by the cyanmethemoglobin method?
a. Increased lipids
b. Elevated WBC count
c. Lyse-resistant RBCs
d. Fetal hemoglobin
d. Fetal hemoglobin
- A patient has a hemoglobin level of 8.0 g/dL. According to the rule of three, what is the expected range for the hematocrit?
a. 21% to 24%
b. 23.7% to 24.3%
c. 24% to 27%
d. 21% to 27%
d. 21% to 27%
- Calculate the MCV and MCHC for the following values: RBCs 5 5.00 3 1012/L HGB 5 9 g/dL HCT 5 30% MCV (fL) MCHC (g/dL)
a. 30 18
b. 60 30
c. 65 33
d. 85 35
b. 60 30
- What does the reticulocyte count assess?
a. Inflammation
b. Response to infection
c. Erythropoietic activity of the bone
marrow
d. Ability of red blood cells to form rouleaux
c. Erythropoietic activity of the bone
marrow
- For a patient with the following test results, which measure of bone marrow red blood cell production provides the most accurate information? Observed
reticulocyte count 5 5.3% HCT 5 35%
Morphology—moderate polychromasia
a. Observed reticulocyte count
b. Corrected reticulocyte count
c. RPI
d. ARC
c. RPI
- Given the following values, calculate the RPI: Observed reticulocyte count 5 6% HCT 5 30%
a. 2
b. 3
c. 4
d. 5
a. 2
- Which of the following would be associated with an elevated ESR value?
a. Microcytosis
b. Polycythemia
c. Decreased globulins
d. Inflammation
d. Inflammation
- Which printout lets the end user know at a glance that the results are acceptable and no manual work needs to be performed?
a. CELL-DYN Sapphire
b. UniCel DxH 800
c. ADVIA 2120i
d. Sysmex XN-series
d. Sysmex XN-series
- Which instrument printout has a system flag on the platelet count?
a. CELL-DYN Sapphire
b. UniCel DxH 800
c. ADVIA 2120i
d. XN-series
a. CELL-DYN Sapphire
- What do you suspect is the cause of the variation in platelet counting among the four instruments?\
a. Different instruments have different levels
of sensitivity.
b. All instruments use the same principle for
counting platelets.
c. Some instruments are susceptible to
false-positive platelet flagging under certain
conditions.
d. Different instruments use different
thresholds to capture and count
platelets.
d. Different instruments use different
thresholds to capture and count
platelets.
- Based on the overall flagging for this specimen on each instrument, should a manual differential count be performed for this patient?
a. Yes, because immature granulocytes are
present in the specimen.
b. Yes, because the WBC scatterplots are
abnormal.
c. No, because each differential count is
complete with no system or morphology
flags.
c. No, because each differential count is
complete with no system or morphology
flags.
- A patient peripheral blood film demonstrates agglutinated RBCs, and the CBC shows an elevated MCHC. What other parameters will be affected by the agglutination of the RBCs?
a. MCV will be decreased and RBC count
will be increased.
b. MCV will be decreased and RBC count
will be decreased.
c. MCV will be increased and RBC count
will be decreased.
d. MCV will be increased and RBC count will
be increased.
c. MCV will be increased and RBC count
will be decreased.
- Match the cell-counting methods listed with the appropriate definition:
C. Impedance - Involves detection and
measurement of changes in electrical current
between two electrodes
B.RF - Uses high voltage electrical waves to
measure the internal complexity of cells
A. Optical Scatter - uses diffraction,reflection and
refraction of light waves
- Low-voltage DC is used to measure:
a. Cell nuclear volume
b. Total cell volume
c. Cellular complexity in the nucleus
d. Cellular complexity in the cytoplasm
b. Total cell volume
- Orthogonal light scatter is used to measure:
a. Cell volume
b. Internal complexity of the cell
c. Cellular granularity
d. Nuclear density
b. Internal complexity of the cell
- On the Beckman Coulter instruments, hematocrit is a calculated value. Which of the following directly measured parameters is used in the calculation of
this value?
a. RDW
b. Hemoglobin
c. MCV
d. MCHC
c. MCV
- Match each instrument listed with the technology it uses to determine WBC differential counts.
B. Abbott CELL-DYN Sapphire - MAPSS
technology
C. Sysmex XN-1000 - Forward&side light
scatter
D. Siemens ADVIA 2120i -
Peroxidase-staining
A. Beckman Coulter UniCel DxH 800 -
Volume, conductivity
- A laboratory science student consistently makes wedge technique blood films that are too long and thin. What change in technique would improve the films?
a. Increasing the downward pressure on the pusher slide
b. Decreasing the acute angle of the
pusher slide
c. Placing the drop of blood closer to the
center of the slide
d. Increasing the acute angle of the pusher
slide
b. Decreasing the acute angle of the
pusher slide
- When a blood film is viewed through the
microscope, the RBCs appear redder than normal, the neutrophils are barely visible, and the eosinophils are bright orange. What is the most likely cause?
a. The slide was overstained.
b. The stain was too alkaline.
c. The buffer was too acidic.
d. The slide was not rinsed adequately.
c. The buffer was too acidic.
- A stained blood film is held up to the light and observed to be bluer than normal. What microscopic abnormality might be expected on this film?
a. Rouleaux
b. Spherocytosis
c. Reactive lymphocytosis
d. Toxic granulation
a. Rouleaux
- A blood film for a very anemic patient with an RBC count of 1.25 3 1012/L shows an average of seven platelets per oil immersion field. Which of the following values most closely correlates with the
estimate per microliter?
a. 14,000
b. 44,000
c. 140,000
d. 280,000
b. 44,000
- A laboratorian using the 403 objective lens sees the following numbers of WBCs in 10 fields: 8, 4, 7, 5, 4, 7, 8, 6, 4, 6. Which of the following WBC counts most closely correlates with the estimate?
a. 1.5 3 109 /L
b. 5.9 3 109 /L
c. 11.8 3 109 /L
d. 24 3 109 /L
c. 11.8 3 109 /L
- A blood film for a patient with a normal RBC count has an average of 10 platelets per oil immersion field. Which of the following values best correlates with the estimate per microliter?
a. 20,000
b. 100,000
c. 200,000
d. 400,000
c. 200,000
- What is the absolute count (3109 /L) for the lymphocytes if the total WBC count is 9.5 3 109 /L and there are 37% lymphocytes?
a. 3.5
b. 6.5
c. 13
d. 37
a. 3.5
- Which of the following blood film findings indicates EDTAinduced pseudothrombocytopenia?
a. The platelets are pushed to the feathered end.
b. The platelets are adhering to WBCs.
c. No platelets at all are seen on the film.
d. The slide has a bluish discoloration when
examined microscopically.
b. The platelets are adhering to WBCs.