Chapter 1: 1.4 Cell Culture Flashcards
What are the two types of eukaryotic cell lines?
- Primary cell culture
- Transformed cell line
State term for:
Growing eukaryotic cells
Tissue culture
Describe:
Primary cell culture
- Healthy cells taken directly from an individual
- Have a limited number of times it can divide
- Often grow/divide very slowly
Give example(s) of cells for Primary Cell Culture
Liver cells, brain cells etc.
Describe:
Transformed cell line
- Cancerous cell lines
- Immortal (unlimited cell division)
- Divide rapidly
Give example(s) of cells for Transformed Cell Line
HeLa cells (cervical cancer cells)
Define:
Confluency
Measure of how dense the cells have grown
True or False:
Transformed cell lines reach confluency very slowly
False, they reach confluency very quickly
How can confluency be seen in images?
100% confluency produces darker images
List:
The four elements of growth conditions for eukaryotic cell cultures
- Media: Culture nutrients needed for the cells
- Container: May have a surface to allow cells to adhere if they are adherent cells
- Environment: Appropriate temperature, oxygem availability, 5% CO2 for survival and cell division
- Sterile: Prevent microorganisms from contamination
In eukaryotic cell cultures:
Cells have to be adherent
(True or False)
False, cells can be free-floating or adherent
In prokaryotic cultures:
Where are bacterial cultures grown?
- On solid media as isolated colonies of bacteria
- In liquid media as suspensions of bacterial cells
In eukaryotic cell cultures:
Why must microorganisms not be allowed to grow in eukaryotic cell cultures?
Microorganisms grow faster and can infect eukaryotic cells
In prokaryotic cultures:
Describe the media
- Contains key nutrients for bacterial growth
- Will be harder if it contains agar
Bacteria divide very ——- (—— than eukaryotic cell)
- Rapidly
- Faster
