Ch7 - The Laboratory Animal Environment

Question 1

differences between isolation room, quarentine, and bioexclusion

Question 2

What species prefers a cooler room?

Answer 2

Rabbits

Question 3

What room ventilation rate is recommended by the Guide?

Answer 3

10-15 exchanges of fresh air per hour

Question 4

Which rooms would likely be kept at positive pressure?

Answer 4

Surgical suites, bioexclusion rooms, “reverse isolation” rooms

Question 5

The macroenvironment is to ______________ as
the microenvironment is to _______________.

Answer 5

entire room; inside the animal’s cage

Question 6

Which rooms would likely be kept at negative pressure?

Answer 6

Biocontainment rooms, isolation rooms, animal quarantine rooms

Question 7

Which factor has the greatest effect on the amount of dissolved oxygen in water?

Answer 7

Temperature

Question 8

High humidity in animal rooms may cause what negative consequence?

Answer 8

Increased feed spoilage and mold

Question 9

(HIGH/LOW) humidity can cause ringtail in mice.

Answer 9

Low humidity

Question 10

What type of room is used to house animals that have been infected with a pathogen? What pressure is this room kept at?

Answer 10

Isolation/biocontainment room; negative pressure

Question 11

A room under POSITIVE pressure ventilation means that there is (MORE/LESS) air in the room, therefore when the door is open, air flows (IN/OUT). This prevents (INSIDE/OUTSIDE) contaminants from coming into the room.

Answer 11

more; out; outside

Question 12

A room under NEGATIVE pressure ventilation means that there is (MORE/LESS) air in the room, therefore when the door is open, air flows (IN/OUT). This prevents (INSIDE/OUTSIDE) contaminants from going out of the room.

Answer 12

less; in; inside

Question 13

What is water hardness?

Answer 13

The amount of calcium and magnesium ions present in water

Question 14

Water used in aquatic housing systems is generally treated by which method before use?

Answer 14

Reverse osmosis

Question 15

What type of room is used to collect blood samples?

Answer 15

Procedure room

Question 16

How far from the wall should feed be stored?

Answer 16

More than 6 inches

Question 17

What waste gases are prevented from accumulating when animal rooms are properly ventilated?

Answer 17

Ammonia and CO2

Question 18

True or False: Aquatic housing rooms should must be able to structurally support the total weight and distribution of water in the housing systems

Answer 18

True

Question 19

Where can housekeeping supplies be stored to minimize the risk of chemical contamination?

Answer 19

In a designated room for equipment and supply storage

Question 20

What type of room is used to house pathogen-free animals? What pressure is this room kept at?

Answer 20

Bioexclusion barrier room; positive pressure

Question 21

What is a bioexclusion room? What pressure is this room kept at?

Answer 21

Barrier prevents entry of all infections agents from getting into a room; positive pressure

Question 22

What is a biocontainment room? What pressure is this room kept at?

Answer 22

Barrier prevents pathogens in the room from being released into the external environment or adjacent animal populations; negative pressure

Question 23

What is a conditioning/acclimation room? Why are incoming animals placed here? What pressure is this room kept at?

Answer 23

Incoming animals are housed in quarantine rooms since they have an unknown health status, where they begin to adjust to new surroundings. This is under negative pressure.

Question 24

True or False: rodents are generally housed in microisolation cages where you can adjust the environmental conditions and ventilation exchange rates can be specifically altered

Answer 24

True

Question 25

What kinds of studies might occur in a negative pressure room?

Answer 25

Studies with hazardous agents, or studies with animals infected with a pathogen

Question 26

What are some negative effects that animals might experience if their daily lighting schedule is changed?

Answer 26

stress, changes in animal physiology that could affect research, decreased breeding efficiency

Question 27

The relative humidity in animal rooms is between ___ - ___ %

Answer 27

30% - 70%

Question 28

What types of animals are especially light sensitive?

Answer 28

Albino animals

Question 29

What species of lab animals require a warm room (68-79F)?

Answer 29

Mouse, rat, hamster, gerbil, guinea pig (rodents)

Question 30

What type of aquatic holding system has the potential for spreading water-borne pathogens across several tanks?

Answer 30

Recirculating systems (share the same water)

Question 31

What is a closed or static aquatic holding system? List an example.

Answer 31

Water is static; only added to maintain a certain volume
Ex: fish bowl, pond

Question 32

What is a flow-through aquatic holding system?

Answer 32

fresh water flows continuously through the system without recirculating

Question 33

What is a recirculating aquatic holding system?

Answer 33

Existing tank water is filtered and reused; most efficient for water consumption and waste removal

Question 34

Tank water must have sufficient quantities of _____ for fish to breathe

Answer 34

Oxygen

Question 35

A waste product that can be toxic and must be removed from the tank water is _______.

Answer 35

Ammonia

Question 36

Maintaining low levels of ______ limits the growth of algae on tank surfaces.

Answer 36

Light

Question 37

_____ is the measure of the acidity or alkalinity of the tank water.

Answer 37

pH

Question 38

_____ is the amount of dissolved salts and minerals in the tank water.

Answer 38

Specific gravity

Question 39

____ is the amount of calcium or magnesium ions in the tank water.

Answer 39

Hardness