Ch5 - Facility Equipment Flashcards
What non-chemical methods are often used to reduce contamination in animal drinking water?
Reverse osmosis and UV radiation
Which type of sanitization equipment can decontaminate a room using a disinfectant without manually washing or scrubbing surfaces?
Misters and Foggers
What are tissue digesters used for?
Carcass disposal
Which biosafety cabinets (class) are known as “glove boxes”, which are airtight and used with high-risk biological agents?
Class III Biosafety Cabinet
Cage Washer
uses high temps and harsh chemicals to sanitize
Tunnel Washer
processed soiled small equipment (ex. rat cages, bottles, feeders)
Rack Washer
used for large equipment (ex. NHP cages, large cage racks)
Cabinet Washer
similar to a dish washer
Bottle Washer
strictly for washing and processing animal drinking bottles and glassware
Pressure Washer
portable machine that sprays water at high temp; used in cage wash, large animal facilities/rooms that do rinse-down
Decontamination Mister
spray disinfectants and sterilants as a fine mist (used in dry conditions)
Decontamination Fogger
used to remove harmful microorganisms in rooms instead of manually washing and scrubbing surfaces (used in dry conditions)
Foamer
hand-held devices used to clean hallways, wash rooms, large animal housing rooms
Which biosafety cabinet (class) draws in room air though a filter, which protects the exterior environment, but not what’s inside the cabinet?
Class I Biosafety Cabinet
Which biosafety cabinet (class) filters the air coming into the cabinet and the air going out into the exterior environment?
Class II Biosafety Cabinet
Why would it be better to use an autoclave to sterilize equipment (surgical instruments, animal housing, etc.) rather than using an incinerator?
Autoclaves use steam at a high pressure and high temp to sterilize items, whereas an incinerator burns trash or tissues into ash.
Foamers aerate what two ingredients into a sanitizing foam?
Water and disinfectant
Chemical fume hoods do not protect:
animals from exposure to pathogens in the air
What does the HEPA filter that’s used in change stations or laminar flow cabinets stand for?
High efficiency particulate air
When testing the efficacy of autoclave sterilization, what does it mean when spores SURVIVE an autoclave cycle?
Sterilization was not achieved
What item should never be autoclaved?
Radioactive material
Large ovens that burn tissues, trash and other materials to ash are called ______. How is waste from this machine disposed of?
Incinerators dispose of waste by flushing it down a sanitary sewer
Why should a vacuum in an animal facility have a HEPA filter?
It minimizes the spread of dust and allergens
What type of feeder is commonly used for pelleted rabbit and guinea pig feeds?
J-shaped clip-on feeder
What is the advantage of using an autoclave over other sterilization methods?
It is fast, reliable, and relatively inexpensive
True or False: Biological safety cabinets require yearly certification to ensure that they are working properly
True
Why are there safety features in cage washers, such as door release mechanisms?
The high temps used in cage wash cycles can be lethal
Autoclaves
Sterilizes instruments, equipment, and glass items using high temperature, superheated steam, and pressure
What are 3 methods used to test for residual microorganisms following decontamination of a room?
Inoculating RODAC plates; PCR testing of plenum and air filter dust; ATP measurement swabs
To properly autoclave a material, the temp and pressure within the autoclave must be maintained for a certain period of time. What 3 characteristics of the material affect this required time?
type of material; size of material; level of contamination
List the three methods that are used to check the effectiveness of an autoclave cycle.
Chemical, biological and mechanical
A Class III BSC protects the user from _________, while a fume hood protects the user from _______.
exposure to pathogenic microorganisms; chemical fumes
What chemicals are often added to animals’ drinking water to reduce bacterial contamination?
Acid and chlorine
