Ch5 - Facility Equipment

Question 1

What non-chemical methods are often used to reduce contamination in animal drinking water?

Answer 1

Reverse osmosis and UV radiation

Question 2

Which type of sanitization equipment can decontaminate a room using a disinfectant without manually washing or scrubbing surfaces?

Answer 2

Misters and Foggers

Question 3

What are tissue digesters used for?

Answer 3

Carcass disposal

Question 4

Which biosafety cabinets (class) are known as “glove boxes”, which are airtight and used with high-risk biological agents?

Answer 4

Class III Biosafety Cabinet

Question 5

Cage Washer

Answer 5

uses high temps and harsh chemicals to sanitize

Question 6

Tunnel Washer

Answer 6

processed soiled small equipment (ex. rat cages, bottles, feeders)

Question 7

Rack Washer

Answer 7

used for large equipment (ex. NHP cages, large cage racks)

Question 8

Cabinet Washer

Answer 8

similar to a dish washer

Question 9

Bottle Washer

Answer 9

strictly for washing and processing animal drinking bottles and glassware

Question 10

Pressure Washer

Answer 10

portable machine that sprays water at high temp; used in cage wash, large animal facilities/rooms that do rinse-down

Question 11

Decontamination Mister

Answer 11

spray disinfectants and sterilants as a fine mist (used in dry conditions)

Question 12

Decontamination Fogger

Answer 12

used to remove harmful microorganisms in rooms instead of manually washing and scrubbing surfaces (used in dry conditions)

Question 13

Foamer

Answer 13

hand-held devices used to clean hallways, wash rooms, large animal housing rooms

Question 14

Which biosafety cabinet (class) draws in room air though a filter, which protects the exterior environment, but not what’s inside the cabinet?

Answer 14

Class I Biosafety Cabinet

Question 15

Which biosafety cabinet (class) filters the air coming into the cabinet and the air going out into the exterior environment?

Answer 15

Class II Biosafety Cabinet

Question 16

Why would it be better to use an autoclave to sterilize equipment (surgical instruments, animal housing, etc.) rather than using an incinerator?

Answer 16

Autoclaves use steam at a high pressure and high temp to sterilize items, whereas an incinerator burns trash or tissues into ash.

Question 17

Foamers aerate what two ingredients into a sanitizing foam?

Answer 17

Water and disinfectant

Question 18

Chemical fume hoods do not protect:

Answer 18

animals from exposure to pathogens in the air

Question 19

What does the HEPA filter that’s used in change stations or laminar flow cabinets stand for?

Answer 19

High efficiency particulate air

Question 20

When testing the efficacy of autoclave sterilization, what does it mean when spores SURVIVE an autoclave cycle?

Answer 20

Sterilization was not achieved

Question 21

What item should never be autoclaved?

Answer 21

Radioactive material

Question 22

Large ovens that burn tissues, trash and other materials to ash are called ______. How is waste from this machine disposed of?

Answer 22

Incinerators dispose of waste by flushing it down a sanitary sewer

Question 23

Why should a vacuum in an animal facility have a HEPA filter?

Answer 23

It minimizes the spread of dust and allergens

Question 24

What type of feeder is commonly used for pelleted rabbit and guinea pig feeds?

Answer 24

J-shaped clip-on feeder

Question 25

What is the advantage of using an autoclave over other sterilization methods?

Answer 25

It is fast, reliable, and relatively inexpensive

Question 26

True or False: Biological safety cabinets require yearly certification to ensure that they are working properly

Answer 26

True

Question 27

Why are there safety features in cage washers, such as door release mechanisms?

Answer 27

The high temps used in cage wash cycles can be lethal

Question 28

Autoclaves

Answer 28

Sterilizes instruments, equipment, and glass items using high temperature, superheated steam, and pressure

Question 29

What are 3 methods used to test for residual microorganisms following decontamination of a room?

Answer 29

Inoculating RODAC plates; PCR testing of plenum and air filter dust; ATP measurement swabs

Question 30

To properly autoclave a material, the temp and pressure within the autoclave must be maintained for a certain period of time. What 3 characteristics of the material affect this required time?

Answer 30

type of material; size of material; level of contamination

Question 31

List the three methods that are used to check the effectiveness of an autoclave cycle.

Answer 31

Chemical, biological and mechanical

Question 32

A Class III BSC protects the user from _________, while a fume hood protects the user from _______.

Answer 32

exposure to pathogenic microorganisms; chemical fumes

Question 33

What chemicals are often added to animals’ drinking water to reduce bacterial contamination?

Answer 33

Acid and chlorine