Ch. Eight: Microbial Genetics Flashcards
1
Q
DNA Helicase
A
- unwinds double-stranded DNA
2
Q
DNA Gyrase
A
- stabilize; relaxes supercoiling ahead of the replication fork
3
Q
Leading Strand
A
- DNA polymerase: adds new nucleotides, winds back
- result: 5’-3’ and 3’-5’
4
Q
Laging Strand
A
- RNA polymerase: form RNA primer
- DNA polymerase: adds new nucleotides but in short segments
- “Okazaki” fragments (few-1000 nucleotides)
- DNA ligase: fills gaps
- result: 3’-5’ and 5’-3’
- RNA primer are digested by DNA polymerase
5
Q
Difference In Bacteria
A
- one circular vs linear
- origin of replication and termination of replication
- two forks vs one
