ch 9 molecular bio n DNA synthesis Flashcards
what is the general structure of DNA?
A nucleotide with DPN
- dbl helix and twisted ladder like
- deoxyribose (sugar
- phosphate group
- Nitrogenous Base
what are the types of N Bases and what are they?
Purines: Adenine & Guanine
Pyridimines: Thymine, Cytosine
what are the primes in DNA that are at the end of each strand
3’ and 5’
What is the general Structure of RNA
- single stranded
- only has ribose (not deoxy like dna)
- instead of Thymine, it has uracil to be paired with A
What are the types of RNA? explain their funcitons
MTR
- messanger - mRNA used for translating amino acids
- transfer - brings amino acids to mRNA in translation
- ribosomal - links appropriate aas
what is the Semiconservative process?
- used in DNA Replication
- when the single DNA strand is used to make the template (new) DNA that will be used for Transcription
DNA Helicase
opens the DNA dbl helix up
DNA Polymerse
enzyme that adds the complimentaty N Bases making the new Template
RNA Primer
Initiates the start seqyuence of the new DNA
Leading Strand (DNA)
the new strand that is continously synthesized
Okazaki Fragments
- small fragments formed in the lagging strand behind the Leading
The Central Dogma
- “prophecy”
DNA –> RNA–> Protein
Generally, what happens during Transcription and Translation?
Transcription - where DNA is copied n mRNA is created
Transaltion - mRNA is used to make amino aicds then proteins
RNA Polymerase
- an enzyme transcribes DNA to make mRNA template
where does Protein Synthesis occur in both Eukaryotes and Prokaryotes
Euk - in nucleus for transcription then in cytoplasm for translation
Prok - all in cytosplasm
Describe what happens during Transcription Initiation
- the DNA is unwinded
- RNA Polymerase pulls up at one strand of DNA and Promotor Sequence directs the start of synthesis
Describe what happens during Transcription Elongations
- RNA Poly does its thing and transcribes complementary N Bases using DNA template strand to then create mRNA
What happens in TRanscription Termination
- mRNA leaves nucleus for cytoplasm and translation
What does Translation need?
- mRNA
- tRNA
- some enzymes
Which type of Ribosome is used for Prot Syntheiss
- large subunit
what does the tRNA do?
- brings the amino acids to the growing chain (mRNA()
what is a Codon
the information that comes in 3’s that mRNA has
what is an Anticodon?
what the tRNA pairs up with the mRNA’;s codon