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Microbioloogy Me > Ch 36 Clinical Microbiology and Immunology > Flashcards

Ch 36 Clinical Microbiology and Immunology Flashcards

1
Q

Standard microbiological practices include all of the following except
A. Work space should be disinfected only when contaminating spills occur.
B. Eating, drinking, manipulation of contact lenses, and the use of cosmetics, gum, and tobacco products are strictly prohibited in the lab.
C. Lab personnel should know how to use the emergency eyewash and shower stations.
D. Hair longer than shoulder length should be tied back.

A

A. Work space should be disinfected only when contaminating spills occur.

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2
Q

Which of the following is not a guideline on selection, collection, and handling of clinical specimens?
A. A small quantity of specimen should be obtained.
B. The specimen selected should be representative of the infectious disease process.
C. The specimen should be forwarded promptly to the clinical laboratory.
D. Attention must be given to avoid contamination of the specimen.

A

A. A small quantity of specimen should be obtained.

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3
Q 
Which of the following tests can be used to distinguish Escherichia from Enterobacter?  
A.  IMVIC 
B.  ONPG test 
C.  citrate utilization 
D.  starch hydrolysis
A

A. IMVIC

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4
Q

The clinical microbiology laboratory can provide preliminary identification of microorganisms based on
A. microscopic examination of specimens.
B. growth or biochemical characteristics.
C. immunologic techniques that detect microbial antigens.
D. all of the choices

A

D. all of the choices

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5
Q 
Which of the following can be examined with an ordinary bright-field microscope? 
A. Wet mounts.
B. Heat-fixed specimens.
C. Chemical-fixed specimens.
D. All of the choices.
A

D. All of the choices.

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6
Q 
Which of the following cell cultures consists of cells derived directly from kidney tissues? 
A. Continuous cell cultures.
B. Semicontinuous cell cultures.
C. Primary cultures.
D. All of the choices.
A

C. Primary cultures.

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7
Q 
Which of the following cell cultures consists of transformed cells? 
A. Continuous cell cultures.
B. Semicontinuous cell cultures.
C. Primary cultures.
D. All of the choices.
A

A. Continuous cell cultures.

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8
Q

Viral replication in cell cultures can be detected by
A. the observation of cytopathic effects.
B. hemadsorption.
C. gram stain.
D. the observation of cytopathic effects and hemadsorption.

A

D. the observation of cytopathic effects and hemadsorption.

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9
Q 
Which of the following sites could be used when inoculating hen's eggs with an infectious agent? 
A. The allantoic cavity.
B. The amniotic cavity.
C. The chorioallantoic membrane.
D. All of the choices.
A

D. All of the choices.

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10
Q 
Which of the following should be isolated only by specialized research laboratories? 
A. mycoplasmas
B. rickettsias
C. chlamydiae
D. all of the choices
A

B. rickettsias

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11
Q 
If one is looking for intracellular inclusion bodies, one would suspect the microorganism to be a 
A. mycoplasma.
B. rickettsia.
C. chlamydia.
D. protozoan.
A

C. chlamydia.

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12
Q 
Which of the following can take weeks to produce obvious growth on routine culture media?  
A.  mycoplasmas 
B.  rickettsias 
C.  chlamydiae 
D.  protozoa
A

A. mycoplasmas

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13
Q 
Ten percent Calcofluor White stain is often used in wet mounts of  
A.  bacteria. 
B.  parasites. 
C.  molds. 
D.  protozoa.
A

C. molds.

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14
Q 
In the clinical laboratory, which of the following is (are) used as a rapid method of identification? 
A. Manual biochemical kit systems.
B. Mechanized/automated systems.
C. Immunologic systems.
D. All of the choices.
A

D. All of the choices.

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15
Q 
An example of miniaturized commercial rapid bacterial identification system based on biochemical analysis is the  
A.  API 20E system. 
B.  Widal test. 
C.  Kirby-Bauer test. 
D.  all of the choices
A

A. API 20E system.

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16
Q 
Which of the following media will permit the growth of one group of bacteria while inhibiting the growth of some other groups? 
A. differential
B. selective
C. enrichment
D. characteristic
A

B. selective

17
Q 
The most common method used by clinical microbiology labs for identifying bacteria are 
A. biochemical.
B. morphological.
C. PCR.
D. DNA fingerprinting.
A

A. biochemical

18
Q 
Which of the following is not used to culture viruses? 
A. Growth on artificial media.
B. Growth in cell cultures.
C. Growth in embryonated hen's eggs.
D. Growth in whole animals.
A

A. Growth on artificial media.

19
Q

Which of the following is useful in the identification of bacteria?
A. Source of the culture specimen.
B. Growth patterns on selective and differential media.
C. Hemolytic, metabolic, and fermentative properties.
D. All of the choices.

A

D. All of the choices.

20
Q 
Which of the following is routinely used to detect spirochetes in skin lesions in early syphilis?  
A.  bright-field microscopy 
B.  phase-contrast microscopy 
C.  dark-field microscopy 
D.  DNA fingerprinting
A

C. dark-field microscopy

21
Q 
Which of the following is/are used to detect chlamydiae in tissues and cell scrapings? 
A. PCR methods.
B. Microscopy of Giemsa stained samples.
C. Immunofluorescence.
D. All of the choices.
A

D. All of the choices.

22
Q 
Hybridoma cells and their progeny normally produce a(n)  
A.  monoclonal antibody. 
B.  polyclonal antibody. 
C.  radioisotopes. 
D.  antigens.
A

A. monoclonal antibody.

23
Q 
The immunologic technique involving the transfer of proteins separated by electrophoresis to nitrocellulose sheets is  
A.  complement fixation. 
B.  direct immunofluorescence. 
C.  immunoblotting. 
D.  immunoelectrophoresis.
A

C. immunoblotting.

24
Q 
Which of the following uses a radioisotope?  
A.  RIA 
B.  ELISA 
C.  API 20E 
D.  all of these
A

A. RIA

25
Q 
Which of the following uses red blood cells?  
A.  hemagglutination inhibition 
B.  MIC 
C.  ELISA 
D.  RIA
A

A. hemagglutination inhibition

26
Q 
Which of the following tests is an agglutination test for the bacterium causing typhoid fever? 
A. Quellung reaction
B. API 20E system
C. Widal test
D. IMViC
A

C. Widal test

27
Q 
Detection of single or multiple microorganisms based on their cell size is known as  
A.  flow cytometry. 
B.  complement fixation. 
C.  agglutination. 
D.  serotyping.
A

A. flow cytometry.

28
Q 
The immunological reaction that occurs when there is an optimal ratio of antigen and antibody, producing a lattice at the zone of equivalence is known  
A.  immunoblotting. 
B.  immunoelectrophoresis. 
C.  immunodiffusion. 
D.  immunoprecipitation.
A

D. immunoprecipitation.

29
Q 
A colorless substrate acted on by the enzyme portion of the ligand to produce a colored product is known as a  
A.  antibody. 
B.  chromogen. 
C.  antigen. 
D.  serotype.
A

B. chromogen.

Microbioloogy Me (11 decks)

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