Ch 15.1-15.3 Flashcards

1
Q

biosynthesis is aka

A

anabolism

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2
Q

3 things biosyn requires

A

essential nutrients transported into the cell (C, N, P, S, O, H) or metals

energy (couple ATP hydrolysis)

reducing power (NADH, NADPH)

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3
Q

prototrophic

A

microorganism has the same nutritional requirements as it did when isolated in nature

ex. E. coli can make everything it needs

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4
Q

auxotrophic

A

a mutant that only grows when provided a specific nutrient

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5
Q

fastidious

A

a microbe that requires many more nutrients than normal

maybe due to enzyme mutation

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6
Q

is biosynthetic capability related to the complexity of the microbe?

A

yes

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7
Q

unity of biochemistry

A

biosyn pathways discovered in bacteria are often found in all other organisms including plants/animals

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8
Q

are biosyn pathways and their enzymes physically associated in the cell?

A

yes, makes the pathways faster

ordered enzymes and pathways

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9
Q

operons

A

group of genes encoding biosynthetic pathways that are all expressed at once

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10
Q

biosynthetic pathways are linked to ______ _______ pathways

A

central metabolism

and the formation of precursor molecules which make families of molecules

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11
Q

aa families

A

aromatics

aspartate

serine

glutamate

pyruvate

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12
Q

other families

A

nucleotides

phospholipids

cell wall components

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13
Q

biosynthetic pathways can be 3 things

A

single

parallel

branched or convergent

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14
Q

anaplerotic rxns

A

make intermediate metabolites

ex. PEP + CO2 –> oxaloacetate

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15
Q

regulation of biosyn pathways occurs in 2 ways

A

enzyme level (feedback inhibition from buildup of intermediates or end product via allosteric sites)

gene regulation (transcription/translation)

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16
Q

medical uses of biosyn

A

exploit pathways by targeting enzymes or intermediates to kill pathogens

ex. mimics of essential molecules used for biosyn of peptidoglycan in mycobacterium TB drugs

17
Q

other bioproduct polydroxyalkanoates

A

a form of strong polyester made by soil bacteria and synthesized in large amounts by engineered E. coli

make biodegradable sutures

18
Q

gluconeogenesis

A

reverse glycolosis to make glucose or fructose

used when microbes are grown on 2-4C sugars

7 shared enzymes, 4 are used only for gluconeogenesis

19
Q

CO2 fixation by what cycle

A

Calvin cycle

20
Q

Calvin cycle used by what 4 groups

A

plants/algae

oxygenic phototrophs (cyanobacteria)

facultative anaerobic

lithoautotrophs

21
Q

3 steps of Calvin cycle

A

carboxylation by rubisco

reduction

regeneration of ribulose 1,5 bisP

22
Q

rubisco

A

ribulose bisphosphate carboxylase

catalyzes condensation of CO2 to ribulose 1,5 bisP and splitting of unstable 6C intermediate into two 3C PGA molecules

inefficient and slow, very abundant 3CO2/sec

23
Q

regulation of calvin cycle

A

rubisco contained in structures called carboxysomes

24
Q

carboxysomes

A

take up bicarbonate which is converted to CO2 by carbonic anhydrase

then CO2 is fixed by rubisco

25
stoichiometry of calvin cycle
(3) 5C ribulose 1,5 bisP + 3 CO2 --> (6) 3PG (5) 3PG are recycled to ribulose 1,5 bisP (1) 3PG is used to make sugars
26
carbon concentrating mechanism (CCM) in cyanobacteria
different ways CO2/HCO3- brought into cell depending on envir conditions constitutive low affinity CO2 transporters inducible high affinity CO2/HCO3- transporters when nutrients low
27
reductive (reverse) TCA cycle
uses 4-5 ATP to fix (4) CO2 and make (1) oxaloacetate reduction by NADPH or NADH or FADH2 green sulfur bacteria - chlorobium (PSII)
28
reductive acetyl CoA pathway used by what 3 groups
acetogenic bacteria sulfate reducers methanogens
29
reductive acetyl CoA pathway
(2) CO2 condensed by converging pathways to make acetyl group of acetyl CoA carbon monoxide intermediate reducing agent is H2
30
3-hydroxypropionate cycle
green phototrophs that are not chlorobium acetyl CoA + HCO3 and 2 NADPH --> 3-hydroxypropinoate (3) CO2 fixed into 1 pyruvate used for biosynthesis
31
secondary metabolites
made through biosynthetic pathways but arent essential for survival can make survival easier ex. high affinity transporters, antibiotics