Cellular Respiration Flashcards
Types of Respiration
- Aerobic respiration
- Anaerobic respiration
- Heat-generating respiration
- Pentose phosphate pathway
- Respiration of Lipids
- Photorespiration
process that breaks down complex carbon compounds into simpler molecules and simultaneously generates the adenosine triphosphate (ATP) used to power other metabolic processes.
Respiration
Carbon oxidation state goes from ___ to ___ as electrons are removed by NAD+, which is converted to NADH in the process
+0 to +4
What removes the electrons from carbon
NAD+
- without air
- type of cellular respiration that does not use oxygen to produce energy
Anaerobic Respiration
- anaerobic process in which energy can be released from glucose even if oxygen is not available
Fermentation
Types of Fermentation
- Alcoholic fermentation
- Lactic acid fermentation
- also referred to as ethanol fermentation
- occurs in plants and yeast
- Yeast cells perform this type of fermentation that produces the alcohol we drink like beer and wine
Alcoholic fermentation
where alcoholic fermentation occurs
- plants
- yeast
- occurs in animals and humans
- produces lactic acid (lactate)
- used to preserve dairy products, vegetables, and meat
Lactic acid fermentation
- actually a poisonous chemical
- accumulation causes cramps and muscle pain
lactic acid (lactate)
- with oxygen present
- A chemical process in which oxygen is used to make energy from carbohydrates (sugars).
Aerobic respiration
means sweet
glyks
means breakdown/ splitting
lysis
where does glycolysis occur
- cytosol
- plastids
series of reactions that extract energy from glucose by splitting it into two three-carbon molecules called pyruvates
Glycolysis
1st phase of glycolysis
- energy is used instead of produced
- glucose is converted into two molecules of glyceraldehyde 3-phosphate
2nd phase of glycolysis
- energy is produced
- glyceraldehyde 3-phosphate is converted into pyruvate
adds a phosphate into glucose to form glucose 6- phosphate
kinase reaction
converts glucose 6-phosphate into fructose-6-phosphate by rearranging covalent bond
isomerase reaction
Another kinase removes a phosphate group from ATP and gives it to fructose 6-phosphate to form ___ ___.
fructose 1,6-bisphosphate
what is formed after the first kinase reaction in glycolysis
glucose 6-phosphate
what is formed after an isomerase reaction in glycolysis
fructose-6-phosphate
splits the 6-carbon fructose 1,6
bisphosphate into two sugars, DHAP and G3P
lyase reaction
what is produced after lyase reaction that splits the 6-carbon fructose
two sugars:
- DHAP
- G3P
DHAP
dihydroxyacetone phosphate
G3P
glyceraldehyde 3-phosphate
Glyceraldehyde 3-phosphate are___ to 1,3-
bisphosphate by dehydrogenase
oxidized
formed after oxidation of glyceraldehyde 3-phosphate
1,3-biphosphate
a ___ transfers a phosphate from 1,3-bisphosphoglycerate to ADP to form ATP and 3-phosphoglycerate
kinase
what is formed after a kinase transfers a phosphate from 1,3-bisphosphogylcerate to ADP
- ATP
- 3-phosphoglycerate
involves a ___ ___ that moves the phosphate to form 2-phosphoglycerate.
mutase reaction
what is formed after a mutase reaction
2-phosphhoglycerate
A ___ ___ removes water from 2-phosphoglycerate to form phosphoenolpyruvate
lyase reaction
what is formed after a lyase reaction that removes water from 2-phosphoglycerate
phosphoenolpyruvate
a ___ ___ removes the phosphate group from phosphoenolpyruvate and donates it to ADP to form ATP and pyruvate.
kinase reaction
what is formed after a kinase reaction that removes the phosphate group from phosphoenolpyruvate and donates it to ADP
- ATP
- pyruvate
what is formed after glycolysis of one glucose molecule
- 2 pyruvate molecule
- 2 ATP
- 2 NADH
- the final pathway of oxidation of glucose, fats, and amino acids
- major source of ATP production in the cells
- large amount of energy is produced after the complete oxidation of nutrients.
citric acid cycle
citric acid cycle is also known as
- tricarboxylic acid cycle
- krebs cycle
citric acid cycle in prokaryotic cells happen where
cytoplasm
citric acid cycle in eukaryotic cells happen where
matrix of mitochondria
citric acid cycle uses ___ and gives out ___ and ___ as products
- uses oxygen
- water and carbon dioxide as products
in citric acid cycle, ___ is converted to ___
ADP to ATP
How many steps are there in citric acid cycle
8 steps
Citric acid cycle step 1
- condensation of acetyl CoA with oxaloacetate
- form 6C citrate
- coenzyme A released
step 1 of citric acid cycle is catalyzed by what
citrate synthase
Citric acid cycle step 2
- citrate converted to isocitrate
step 2 of citric acid cycle is catalyzed by what
aconitase
Citric acid cycle step 3
- isocitrate undergoes dehydrogenation & decarboxylation
- form 5C α-ketoglutarate
- CO2 is released
step 3 of citric acid cycle is catalyzed by what
isocitrate dehydrogenase
isocitrate dehydrogenase is dependent to ___. Because of this, it is converted to ___
- NAD+
- NADH
Citric acid cycle step 4
- α-ketoglutarate undergoes oxidative decarboxylation
- succinyl CoA is formed
step 4 of citric acid cycle is catalyzed by what
α-ketoglutarate dehydrogenase
Step 4:
one molecule of ___ is released and ___ is converted to ___
- CO2 is released
- NAD+ converted to NADH
Citric acid cycle step 5
- succinyl CoA forms succinate
step 5 of citric acid cycle is catalyzed by what
succinyl CoA synthetase
succinyl is coupled with substrate-level phosphorylation of ___ to get ___. Then, it transfers its phosphate to ___ forming ___
- GDP
- GTP
- ADP
- ATP
Citric acid cycle step 6
- succinate is oxidized
- fumarate is formed
step 6 of citric acid cycle is catalyzed by what
succinate dehydrogenase
in step 6 of citric acid cycle, ___ is converted to ___
- FAD
- FADH2
Citric acid cycle step 7
- fumarate converted to malate by addition of one H2O
step 7 of citric acid cycle is catalyzed by what
fumarase
Citric acid cycle step 8
- malate dehydrogenated
- oxaloacetate is formed, and combines to another acetyl CoA and starts new cycle
step 8 of citric acid cycle is catalyzed by what
malate dehydrogenase
in step 8 of citric acid cycle, ___ is removed and get transferred to ___ forming ___
- hydrogens removed
- NAD+ forming NADH
what carries protons and electron to the electron transport chain to generate additional ATP by oxidative phosphorylation
- NADH + H+
- FADH2
process by which ATP synthesis is coupled to the movement of electrons through the mitochondrial electron transport chain and the associated consumption of oxygen
Oxidative phosphorylation
For complete oxidation of one glucose molecule, citric acid cycle yields
- 4 CO2
- 6 NADH
- 2 FADH2
- 2 ATPs
each molecule of NADH can form ___
2-3 ATPs
each FADH2 gives ___ on oxidation in the electron transport chain
2 ATPs
form of vitamin B2 used to restore riboflavin in anemia, migraine, alcoholism, and hyperhomocysteinemia
Flavin mononucleotide (FMN)
- represent the membrane hub where most of the respiratory complexes embed to account for OXPHOS and energy production in the form of adenosine triphosphate (ATP)
- Changes in the number and shape define the respiratory capacity as well as cell viability
Crista lumen
- coenzyme central to metabolism
- consists of two nucleotides joined through their phosphate groups
Nicotinamide adenine dinucleotide (NADH)
- helps mitochondria (small structures in the cell) make energy
- antioxidant that helps prevent cell damage caused by free radicals (highly reactive chemicals)
Ubiquinone
proteins that contain heme as their prosthetic group and whose principal biological function, in the cells of animals, plants, and microorganisms, is electron transport
Cytochromes
- transmembrane molecule found in the mitochondria of eukaryotes and in the cellular space of aerobic prokaryotes
- proton pump that plays a vital role in producing energy, in the form of ATP, via the ETS
Cytochrome oxidase
- electrons are passed from one molecule to another, and energy released in these electron transfers is used to form an electrochemical gradient
- In chemiosmosis, the energy stored in the gradient is used to make ATP
electron transport chain
- transports the substrate for oxidative metabolism directly from the cytosol to the mitochondrial electron transport chain
- essential for glucose-induced activation of mitochondrial metabolism and insulin secretion in adult β-cells
NADH shuttle system
Types of shuttle
- Malate-aspartate shuttle
- Glycerol phosphate shuttle
- essential system used by mitochondria
- NADH in the cytosol powers the conversion of oxaloacetate to malate
- Malate is converted to aspartate and transported back out of the mitochondrion, where it is converted to oxaloacetate again and can repeat the cycle
malate-aspartate shuttle
- byproduct of glycolysis
- important pathway for delivery of cytosolic reducing equivalents into mitochondrial oxidative phosphorylation
- mechanism that regenerates NAD+ from NADH
glycerol phosphate shuttle
- cellular respiration that allows plants produce heat
- takes place in the mitochondria
thermogenic respiration
Ex. of thermogenic plants
- voodoo lily
- skunk cabbage
Thermogenesis in plants
- No proton gradient and no chemiosmosis production of ATP
- Alternative Electron Carriers (do not pump protons during electron transport in mitochondria)
- Energy in NADH is converted entirely to heat
In plants, alternative electron carriers do not interact with ___, ___ and ___
- cyanide (CN-)
- azide (N3-)
- carbon monoxide (CO)
thermogenic/heat-generating respiration is also called ___ ___
cyanide-resistant respiration
- produce sugars that make up DNA and RNA (ribose)
- produce sugars that make up lignin and anthocyanin (erythrose)
- Produce NADPH (Nicotinamide adenine dinucleotide phosphate)
- Occurs in cytosol and plastids
pentose phosphate pathway
where does pentose phosphate pathway occurs
- cytosol
- plastid
the deposition of __ in cell walls make up woody plant parts
lignin
colored water-soluble pigments in vacuoles
anthocyanins
Pentose Phosphate Pathway:
meristematic cells, favors what
ribose production
Pentose Phosphate Pathway:
woody cells, favors what
erythrose production
Pentose Phosphate Pathway:
other cells, favors what
NADPH production
Lipids undergo __ in which the fatty acids are separated from either glycerol of glycerol phosphate
lipolysis
fatty acids are broken down into acetyl CoA by a process called
beta oxidation
activation of fatty acids to become acyl-CoA is catalyzed by what enzyme
acyl-CoA synthetase
Four (4) steps in beta-oxidation
- dehydrogenation
- hydration
- oxidation
- thiolysis (cleavage)
each round of beta-oxidation uses
- 1 FAD+
- 1 NAD+
- 1 H2O
- 1 CoA
each round of beta-oxidation generates
- 1 NADH
- 1 H+
- 1 FADH2
- 1 acetyl coA (last round produces 2)
acetyl coa may be used for synthesis of ___ or may enter __ __ __ and be further respired
- carbohydrates
- citric acid cycle
enzyme that catalyzes lipolysis
lipase
- Refers to a process in plant metabolism where the enzyme RuBisCO oxygenates RuBP, wasting some of the energy produced by photosynthesis
- RuBisCO reacts with oxygen rather than carbon dioxide.
photorespiration
Organelles that participate in photorespiration
- chloroplast
- peroxisome
- mitochondrion
Environmental factors and internal factors that affect rate of respiration
- Temperature
- Lack of Oxygen
- Internal regulation
The cellular respiration in plants increases with an increase in ___ until the point- when a further increase will lead to tissue deterioration.
temperature
Optimal temperature of plants to have the highest enzyme activity
40°C
The ___ ___ ___ in the surrounding atmosphere greatly influence the rate of respiration.
percentage of oxygen
During fruit maturation, respiration usually remains ___ or __ __ until just before the fruit is mature
- steady
- increase gradually
Sudden burst of respiration in fruit maturation is triggered by __ __
endogenous hormones
Within seeds, after an embryo is mature, its respiration ___ dramatically and the seed becomes dormant
- decreases
Tissues that store large amounts of lipids
- oily seeds
- dormant apical meristem
Cells that have metabolism, have high level of __ __
aerobic respiration
Respiration is much higher in ___ season than in ___ season for the other organs
- summer
- winter
respiration usually remains or increase gradually until just before the fruit is mature
fruit maturation
- respiration decreases so dramatically after an embryo is mature
- becomes dormant
within seeds
no respiration occurs even if the embryo is surgically removed and given water, warmth, and oxygen
seeds with true dormant period
Production of ATP by Various Types of Respiration:
Anaerobic respiration
2 ATP -> 2ATP
Production of ATP by Various Types of Respiration:
Glycolysis (Aerobic respiration)
2 ATP -> 2 ATP
2 NADH -> 4 or 6 ATP
Production of ATP by Various Types of Respiration:
Pyruvate/acetyl CoA (Aerobic respiration)
1 NADH -> 3 ATP (2) -> 6 ATP
Production of ATP by Various Types of Respiration:
Citric acid cycle (Aerobic respiration)
1 ATP (2) -> 2 ATP
3 NADH -> 9 ATP (2) -> 18 ATP
1 FADH2 -> 2 ATP (2) -> 4 ATP
Production of ATP by Various Types of Respiration:
Pentose phosphate pathway
2 to 6 NADPH -> 0 to 12 ATP
Production of ATP by Various Types of Respiration:
Heat-generating respiration
none
Production of ATP by Various Types of Respiration:
Lipid respiration
variable
Production of ATP by Various Types of Respiration:
Photorespiration
none
- ratio of volume of carbon dioxide given out and volume of oxygen taken in during respiration
- depends upon respiratory substrates and their oxidation
Respiratory Quotient or Respiratory Ratio
- contain relatively large amounts of oxygen in their molecular structure, less is needed to convert them to carbon dioxide and water
Acids
contain virtually no oxygen
fatty acids
Formula of RQ
RQ = CO2 liberated / O2 consumed
Examples of alcoholic beverages
- beer
- wine
- spirits
- made by fermenting starchy cereal grains, especially barley, wheat, corn, or rice
Beer
by far most common type of grain used in beer
barley
sprouted, dried barley grains
malt
sugar-rich liquid resulting from the mashing process of beer
wort
fermented rice, usually called rice wine
sake
fermented fruit juices that are rich in sugars
wine
for white wines, the __ are removed, but it is mixed with red or rose wines
exocarps
fermentation temperature for white wines
10 - 15°C
fermentation temperature for red wines
25 - 30°C
are made by adding sugar to a bottle of wine that sill has a few live yeast cells in it
- champagnes
- sparkling wines
extra ethanol is added to wine
fortified wine
alcoholic beverages with an ethanol content above 20%
spirits
distillation of gran-based fermentations produces
- whiskeys
- vodka
- gin
results from distillation of grape wine
brandy
distillation of fermented molasses
rum
distilled from fermented juices of agave plants
- tequila
- mescal
fermentation of plant material always produces __ __
ethyl alcohol (ethanol)
Too much ethanol will disrupt the __ in the cell membrane
lipids
- very toxic by itself
- our livers convert it into an even more poisonous substance
methyl alcohol
our livers convert methyl alcohol into
- formaldehyde
- formic acid