Cell Structure and Function 1,2,3 Flashcards
(histology/ cytology)The anatomical study of the microscopic structure of tissues
histology
(histology/ cytology)The study of the structure and function of cells
Cytology
Periodic Acid-Schiff Reaction Detects (glycogen/phophate)
glycogen
Acid Phosphatase Reaction Detects (Phosphatase/Kinase) Enzyme Activity
phosphatase
(Light/electron)Live cells may be examined in a culture dish, but more typically tissues are fixed, sectioned(typically 5-50 micron thick slices), and stained.
light
(Light/electron)Includes bright field, phase contrast, polarized, dark-field, fluorescent, and confocal microscopy.
light
T/f the most common stain is hemotoxylin eosin stain
T
(Hemotoxylin/Eosin) is a basic stain that is deep purple in color; it stains heterochromatin and
nucleoli in the nucleus, and ribosomal RNA. Such structures are said to be (basophilic/eosinophilic)
hemotoxylin , basophilic
(Hemotoxylin/Eosin)is an acidic stain that is usually pink to red in color; it stains cytoplasm. Suchstructures are said to be eosinophilic or (acidophilic/basophilic)
eosin, acidophilic
(Light/electron) travel in wavelengths of approximately 1/2,000 that of visible light
electron
T/F electron microscopy facilitates a more detailed analysis of the morphology of cellular components.
T
T/F Typically, the specimens in electron microscopy are stained with heavy metals that can absorb or deflect the electron beam.
T
(Scanning electron microscopy/Transmitting electron microscopy/Freeze-fracturing) a beam of electrons is passed through the
specimen (similar to bright field microscopy) and only some of the electrons pass through the specimen to the detector, the rest are either absorbed or scattered by different cellular components.
(Scanning electron microscopy
(Scanning electron microscopy/Transmitting electron microscopy/Freeze-fracturing) a beam of electrons is passed across the surface of the specimen and electrons that are deflected by or emitted from the specimen are detected by the detector.
Transmitting electron microscopy
(Scanning electron microscopy/Transmitting electron microscopy/Freeze-fracturing) is a process of sample preparation that uses extreme cold temperature and striking the specimen instead of slicing prescribed thickness sections through the specimen. Fracture planes follow the hydrophobic region within lipid bilayers, revealing the interior of the plasma membrane.
Freeze-fracturing
Freeze fracturing , fracture planes and follow the hydrophobic region within lipid bilayers, revealing the (outer/interior) of the plasma membrane
interior
A. Periodic acid-Schiff (PAS) staining
B. Enzyme histochemistry
C. Immunohistochemical staining
detects carbohydrates by replacing the -OH groups onadjacent carbons on the hexose ring with aldehydes (=O).The aldehyde groups react withSchiff’s reagent [F(SO4H)2] turning it a deep magenta color.
A. Periodic acid-Schiff (PAS) staining
A. Periodic acid-Schiff (PAS) staining
B. Enzyme histochemistry
C. Immunohistochemical staining
uses stains that are activated or change color when cleaved by aparticular enzyme to detect the presence of that enzyme
B. Enzyme histochemistry
A. Periodic acid-Schiff (PAS) staining
B. Enzyme histochemistry
C. Immunohistochemical staining
uses dyes bound to antibodies to detect the presence of specific antigens on and within cells.
C. Immunohistochemical staining
Acid Phosphatase reaction detects the presence and activity of ______
phosphatase enzymes
When phophatases hydrolyze phosphate esters create an (phosphate/ester/methyl/alcohol) pick two
alcohol, phosphate
In a acid phosphatase reaction, phosphatase enzymes are detected by binding lead and then sulfide creating an insoluble (black/red/white) precipitate
black
A. Periodic acid-Schiff (PAS) staining
B. Enzyme histochemistr
C. Immunohistochemical staining
-uses dyes bound to antibodies to detect the presence of specific antigens on and within cells.
C. Immunohistochemical staining
T/F all the organs of thehuman body are composed entirely of 4 types of tissues
T
- Epithelium
- Connective tissue
- Muscle tissue
- Nervous tissue
- Covers body surfaces, lines body cavities, and forms glands. _ are connected to one another to form a continuous sheet.
Epithelium
- Epithelium
- Connective tissue
- Muscle tissue
- Nervous tissue
- Cells secrete an extracellular matrix; unlike epithelium, cells of _ are generally not in direct contact with one another
Connective tissue