11-4-13 Replication and Telomeres

Question 1

T/F mammalian and E. coli replicaiotn processes are similar

Answer 1

T

Question 2

T/F all component of repliaciont and mutations in genes responsible for replication have been characterized in yeast

Answer 2

T

Question 3

-similar replication fork geometry
-mulitprotein replication machine
are the same in (yeast/e coli./mammalian)

Answer 3

e.coli and mammalian

Question 4

Genome- 4.64 million basepairs circular DNA
Single origin of replication
Same polymerase for leading and lagging strand synthesis
describes (e.coli/mammalian)

Answer 4

e. coli

Question 5

23 pairs of linear chromosomes- 3.2 billion basepairs
Multiple origins of replication (~1000)
Same or different polymerases for leading and lagging strand synthesis
describes (e.coli/mammalian)

Answer 5

mammalian

Question 6

single origin of replication

-describes (ecoli/mammalian)

Answer 6

e.coli

Question 7

same polymerase for leadig and lagging strand

-describes (ecoli/mammalian)

Answer 7

e.coli

Question 8

multiple origins of replication

-describes (ecoli/mammalian)

Answer 8

mammalian

Question 9

same or different polymerases for leading and lagging strand synthesis
(e.coli/mammalian)

Answer 9

mammalian

Question 10

RNaseH degrades (DNA/RNA)

Answer 10

RNA

Question 11

T/F okasaki fragments are smaller than E. coli

Answer 11

T

Question 12

polymerase (alpha/beta/gamma) is primase

Answer 12

alpha

Question 13

polymerase (alpha/beta/gamma) is mitochondrial replication

Answer 13

gamma

Question 14

polymerase (alpha/beta/gamma) is repair

Answer 14

beta

Question 15

polymerase sigma and e are both ____

Answer 15

nuclear replication

Question 16

(e.coli/mammalian) replication is cirlcular

Answer 16

e.coli

Question 17

(e.coli/mammalian) replication is linear

Answer 17

mammalian

Question 18

which end will shorten with each replication cycle?

5’ or 3’

Answer 18

5’

Question 19

• coding sequences may be eventualy lost
• senescence or death signal may be triggered
• -these are both consequence of what?

Answer 19

shortening of the 5’ end after each replication cycle/

Question 20

• origin of repliication
• centromere
• telomere
• -these are all requirement for what

Answer 20

chromosome replication

Question 21

(centromere/teleomore) seal the ends of chromosomes

Answer 21

telomere

Question 22

• avoid undesirable fusion
• aboid aberrant recomination
• –(centromere/teleomere)

Answer 22

telomere

Question 23

(centromere/teleomore) attach chromosomes to nuclear envelope

Answer 23

telomere

Question 24

telomeres have (sepa/hexa)meric repeats

Answer 24

hexameric

Question 25

___ makes teomeric repeats

Answer 25

telomrase

Question 26

what is a telomerase?

Answer 26

protein + RNA

Question 27

what type of activity does the protein portion of the temoerase do?

Answer 27

reverse transcriptase

Question 28

telomerase is (DNA/RNA) dependent DNA polymerase

Answer 28

RNA

Question 29

which end does telomerase extend?

Answer 29

3'

Question 30

telomere repeats from G (quartets/triplets)

Answer 30

quartets

Question 31

what is the name of the tail made by the telomerase (A tail/G tail)

Answer 31

G-tail

Question 32

(somatic/germ) cells have detectable activity

Answer 32

germ

Question 33

(somatic/germ) cells have no detecable activiyt

Answer 33

somatic

Question 34

fibroblast from the (young/elderly) have shorter fibroblasts

Answer 34

elderly

Question 35

- replicative sensecense - apoptosis - -are induced by (long/short) telomeres

Answer 35

short

Question 36

T/F in human cnacer there is more telomerase activity than in normal tissue

Answer 36

T

Question 37

GRN163L and G-quadruplex stabilizing agens are thoguh to (activate/block) the replicative portention of cancer cells

Answer 37

block

Question 38

Viruses (have/don't have) a cell structure or metabolism, but they (are/aren't) capable of reproducing viruses

Answer 38

dont have, aren't

Question 39

____ is an inert virus particle outside of teh cell host

Answer 39

virion

Question 40

virus genomes are (single/double) DNA and RNA

Answer 40

single double

Question 41

T/F viral nucleic acids are replicated only in host cells using host cell machinery

Answer 41

T

Question 42

Replication of viruses involves : what three things?

Answer 42

synthesis of genome, translation , transcription

Question 43

Viral genomes (are/aren't) variable in size organization and replication patterns

Answer 43

are

Question 44

HIV has A) 9749 nucleotides 9 genes 15 proteins B) 9749 nucleotides 15 genes 9 proteins

Answer 44

A

Question 45

AZT inhibits (reverse transcriptiase/integrase)

Answer 45

reverse transcriptase

Question 46

bacterial gyrase inhibitors are used in (antibiotics/chemotherapy)

Answer 46

antibiotics

Question 47

topoisomerase inhibitors are used for (antibiotics/chemotherapy)

Answer 47

chemotherapy

Question 48

5-Florouracil inhibitor of (DNA replication/reverse transriptase) used for cancer chemotherapy

Answer 48

DNA replication

Question 49

5-florouriacil is an analogue for uracil and ___

Answer 49

thymine

Question 50

Capecitabine is a orally administered chemotherapeutic agent which is converted to (5-florouracil/AZT)

Answer 50

5-Florouracil