11.06.13 Molecular Biology Transcription

Question 1

What are the two difference between DNA and RNA?

Answer 1

RNA has ribose
DNA has deoxyribose

RNA has Uracil
DNA has thymine

Question 2

RNA is mostly (double/single) stranded

Answer 2

single

Question 3

hepatits delta virus come from (DNA/RNA)

Answer 3

RNA

Question 4

T/F RNA as a genome

Answer 4

T

Question 5

T/F RNA is carrier of information

Answer 5

T

Question 6

T/F RNA deals with protein synthesis

Answer 6

T

Question 7

T/F RNA has a catalytic funciton

Answer 7

T

Question 8

(rRNA/tRNA/mRNA) most abundant

Answer 8

rRNA

Question 9

(rRNA/tRNA/mRNA) less abundant

Answer 9

tRNA

Question 10

(rRNA/tRNA/mRNA) least abundant

Answer 10

mRNA

Question 11

RNA polymerase binds to (active site/promotor region)

Answer 11

promoter region

Question 12

no primer is required

• no proofreadin occurs
• -in (DNA/RNA) trancription

Answer 12

RNA

Question 13

T/F in RNA transcritpion terminates at a hairpin loop

Answer 13

T

Question 14

(e.coli/yeast/simplest polymerase) has a single polypeptide T3 and T7 bacteriophages

Answer 14

simplest polymerase

Question 15

(e.coli/yeast/simplest polymerase) 5 subunits

Answer 15

e.coli

Question 16

(e.coli/yeast/simplest polymerase) 12 subunits

Answer 16

yeast

Question 17

(yeast/E Coli) RNA polymerase has a w subunit

Answer 17

e.coli

Question 18

promotore stength = ____ of transcription

Answer 18

rate

Question 19

T/F sequences of strong promotors are closer to conseus sequence

Answer 19

T

Question 20

(e coli/yeast) 35 sequece , 10 sequence promotor . Start point is oncidered +1

Answer 20

e. coli

Question 21

(core enzyme/holoenzyme) can bind any DNA

Answer 21

core enzyme

Question 22

(core enzyme/holoenzyme) forms and binds a promoter closed complex

Answer 22

holoenzyme

Question 23

(holoenzyme/polymerase) unwinds the DNA open complex

Answer 23

polymerase

Question 24

what is the connection between drug resistance, antibiotics and trascription??(helicase/topoisomerase) inhibitors

Answer 24

topoisomerase

Question 25

what is the connection between drug resistance, antibiotics and trascription??(helicase/topoisomerase) inhibitors

Answer 25

topoisomerase

Question 26

(Novobiocin/Ciprofloxacin)
Quinolone antibiotic Binds to the A subunit of bacterial DNA gyrase.
DNA cleavage and resealing activity impaired.
No sealing of double-strand breaks: DNA damage.

Answer 26

ciprofloxacin

Question 27

(Novobiocin/Ciprofloxacin)

Coumarin antibiotic
inhibits bacterial DNA gyrase by binding to the ATP binding site in its B subunit.
Inhibits its DNA supercoiling activity.
Transcription of many bacterial genes disrupted

Answer 27

novobiocin

Question 28

what are some differences in prokaryotic vs eukaryotic transctiption?

Answer 28

structure of RNA polymerase
promoter sequence
bacteria have operon
eukaryotic genes are individually regulated

Question 29

(eukaryotes/prokaryotes) RNa polymerase does not bind promoter directly

Answer 29

eukaryotes

Question 30

(eukaryotes/prokaryotes) RNa polymerase does not bind promoter directly

Answer 30

eukaryotes

Question 31

(eukaryotes/prokaryotes) RNa polymerase does bind promoter directly

Answer 31

prokaryotes

Question 32

(rifampin/Alpha-amanitin) inhibits e.coli

Answer 32

rifampin

Question 33

(rifampin/Alpha-amanitin) inhibits human mitochondrial

Answer 33

riampin

Question 34

(rifampin/Alpha-amanitin) inhibits RNA pol 1,ii,iii

Answer 34

alpha amanitin

Question 35

(rifampin/alpha-amanitin) Drug blocks the initiation of transcription by binding to a protein pocket near the active site of the β subunit of the bacterial polymerase to block the path of the nascent RNA during initiation

Answer 35

rifampin

Question 36

what are tehe two important parts in a actinomycin D transcription inhibitor

Answer 36

oligopeptide tides, phenoxazone ring

Question 37

actinomycin d block movement of (DNA/RNA) polymerase

Answer 37

RNA

Question 38

phenoxazone ring gts intercalagted between two (CG/AT) pairs

Answer 38

CG