11.06.13 Molecular Biology Transcription Flashcards
What are the two difference between DNA and RNA?
RNA has ribose
DNA has deoxyribose
RNA has Uracil
DNA has thymine
RNA is mostly (double/single) stranded
single
hepatits delta virus come from (DNA/RNA)
RNA
T/F RNA as a genome
T
T/F RNA is carrier of information
T
T/F RNA deals with protein synthesis
T
T/F RNA has a catalytic funciton
T
(rRNA/tRNA/mRNA) most abundant
rRNA
(rRNA/tRNA/mRNA) less abundant
tRNA
(rRNA/tRNA/mRNA) least abundant
mRNA
RNA polymerase binds to (active site/promotor region)
promoter region
no primer is required
- no proofreadin occurs
- -in (DNA/RNA) trancription
RNA
T/F in RNA transcritpion terminates at a hairpin loop
T
(e.coli/yeast/simplest polymerase) has a single polypeptide T3 and T7 bacteriophages
simplest polymerase
(e.coli/yeast/simplest polymerase) 5 subunits
e.coli
(e.coli/yeast/simplest polymerase) 12 subunits
yeast
(yeast/E Coli) RNA polymerase has a w subunit
e.coli
promotore stength = ____ of transcription
rate
T/F sequences of strong promotors are closer to conseus sequence
T
(e coli/yeast) 35 sequece , 10 sequence promotor . Start point is oncidered +1
e. coli
(core enzyme/holoenzyme) can bind any DNA
core enzyme
(core enzyme/holoenzyme) forms and binds a promoter closed complex
holoenzyme
(holoenzyme/polymerase) unwinds the DNA open complex
polymerase
what is the connection between drug resistance, antibiotics and trascription??(helicase/topoisomerase) inhibitors
topoisomerase
what is the connection between drug resistance, antibiotics and trascription??(helicase/topoisomerase) inhibitors
topoisomerase
(Novobiocin/Ciprofloxacin)
Quinolone antibiotic Binds to the A subunit of bacterial DNA gyrase.
DNA cleavage and resealing activity impaired.
No sealing of double-strand breaks: DNA damage.
ciprofloxacin
(Novobiocin/Ciprofloxacin)
Coumarin antibiotic
inhibits bacterial DNA gyrase by binding to the ATP binding site in its B subunit.
Inhibits its DNA supercoiling activity.
Transcription of many bacterial genes disrupted
novobiocin
what are some differences in prokaryotic vs eukaryotic transctiption?
structure of RNA polymerase
promoter sequence
bacteria have operon
eukaryotic genes are individually regulated
(eukaryotes/prokaryotes) RNa polymerase does not bind promoter directly
eukaryotes
(eukaryotes/prokaryotes) RNa polymerase does not bind promoter directly
eukaryotes
(eukaryotes/prokaryotes) RNa polymerase does bind promoter directly
prokaryotes
(rifampin/Alpha-amanitin) inhibits e.coli
rifampin
(rifampin/Alpha-amanitin) inhibits human mitochondrial
riampin
(rifampin/Alpha-amanitin) inhibits RNA pol 1,ii,iii
alpha amanitin
(rifampin/alpha-amanitin) Drug blocks the initiation of transcription by binding to a protein pocket near the active site of the β subunit of the bacterial polymerase to block the path of the nascent RNA during initiation
rifampin
what are tehe two important parts in a actinomycin D transcription inhibitor
oligopeptide tides, phenoxazone ring
actinomycin d block movement of (DNA/RNA) polymerase
RNA
phenoxazone ring gts intercalagted between two (CG/AT) pairs
CG
