11.6.13 Molecular Biology Transcription part 6 Flashcards
Required for general cell functions and cell viability.
enzymes of basic metabolism and proteins for cell structures such as ribosomes.
(house keeping genes/regulated genes)
house keeping genes
cell-type specific genes which are responsible for the differentiated functions of specific cell types. These also include genes which respond to signals from other cells.
hormone/growth factor/stress responsive genes
(house keeping genes/regulated genes)
regulated genes
(prokaryotic/eukaryotes) regulation involves Adjustment of metabolic requirement in response to nutritional environment
prokayrotic
T/F e.coli prefers glucose above all other sugars
T
cAMP is (high/low) when glucose is present
low
DBP carry out (positive/negative) control with CAP-cAMP and (negative/postive) with lac and trp repressor
positive, negative
repressors are (negative/positive) control
negative
activators are (negative/positive) control
positive
class (I, II, III) genese make rRNA
class I
class (I, II, III) genese make mRNA, snRNA
class II
class (I, II, III) genese make tRNA, snRNA
class III
-TATA
-iniatiator
-TATA less and initiator less
are all (promotor/inhibitor)
promotors
in the TATA less and initaor less what is there instead for the promoters to recognize?
GC island
pol (i. ii) it has silencers that repress transcription
II
GTF binds to TATA and INR and positons RNA pol (I, II, III)
II
out of all the TFII which one has helicast ATPAase activity ?
TFIIH
how many TAFs are there
A)15
b)14
c)13
14
TBP is (assymetrical/symmetrical) in its tertiary structure. Either side of the protein reaches into the (minor/major) grove of the DNA to bind and distort promoter DNA structure.
symmetrical, minor groove
whats the structore of activator that binds to a specific DNA sequence?
itsa modular protein
How do activator communicate with pol II?
uses co activator
what type of motif do homeobox protiens have (helix-turn-helix/helix-loop-helix)
helix-turn-helix
name the four types of motifs that DNA binding protein can have
HTH, HLH, ZN finger, leuzine sipper
T/F Hox genes control
- morphology of vertebra
- segmentation of hindbrain
- digits of the limbs
T
How many human genes are there?
A)25,000
B)30,000
C)40,000
25,000
T/F Gene control is dont on a gene-specific or cell specific manner
T
Human disease is linked to pol (I, II, III)
II
VHL is a ____
genetic disorder
VHL binds to subunits (b/C/A/D)
B and C
elongin complex (decreases/increases) the rate of trancription elongation
increases
T/F Mutations in VHL protein prevent its binding to elongin
T
loss of VHL lead to (deactivation/activiation) of angiogneic pathways
activation
VHL mutation -> prevents binding of elongin BC–> and thus inhibits the formation of active ubiquitin ligase00> HIF is not degraded –> tumor growth is sustained
T
GH stands for ?
growth hormone
GH is dependent on binding of trancription factor (VHL/Pit-1)
pit-1
How many amino acids are there in Pit-1
A)291
b)391
c)491
291
(VHL/Huntington’s disease) is an inherited neurodegenerative disease, progressive deterioratoin of motor and cognitive abilities
Huntington’s disease
