cell structure

Question 1

homogenisation

Answer 1

breaking up tissues and opening up cells using4
using blender or homogenising plunger

Question 2

centrifuge machine

Answer 2

spins cell homogenate to seperate organelles based on mass (heavy nucleus goes to bottom first)

Question 3

supernatant

Answer 3

remaining liquid after centrifugation

Question 4

1m to mm

Answer 4

x1000

Question 5

1mm to μm (micrometers)

Answer 5

x 1000

Question 6

1μm to nm (nanometer)

Answer 6

x1000

Question 7

light microscope pros

Answer 7

cheap
easy to use
portable
can view living specimens

Question 8

light microscope cons

Answer 8

low resolution
low magnification
cannot be used in bright places

Question 9

resolution

Answer 9

image clarity
minimum distance between two objects where they can be seen as two seperate objects

Question 10

why does a light microscope have a low resolution

Answer 10

wavelength of light is too long to pass through close organelles
so low resolution

Question 11

magnification defintion

Answer 11

the amount of times larger an object seems compared to its actual size

Question 12

magnification equation

Answer 12

I = AM
image size = actual x magnification

Question 13

TEM: all you need to know

Answer 13

electron microscope that shows cells internal ultrastructure

has the highest magnification and resolution

cannot view living specimens as sample is chemically fixed and dehydrated and put in a vacuum

produces 2d greyscale photomicrograph

Question 14

SEM: all you need to know

Answer 14

shows tissues external topography

has a high mag and res

cannot view living specimens

produces 3d grey scale image

Question 15

eukaryotic cells overview

Answer 15

contains DNA in a membrane bound nucleus
(animal, plants, fungi)