Cell Fractionation Flashcards

1
Q

What is the method for cell fractionation?

A
  • grind in
  • cold water - reduce enzyme activity
    -isotonic- prevents organelles from shrinking or bursting
    -buffer- maintains ph
    -filter out large cell debris but the organelles pass through
    Centrifugation
    Low speed for the heaviest organelles and high speed for the lightest organelles
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