beetroot practical

Question 1

what’s the effect of temperature on movement of pigment out of the betroot cell through membrane

Answer 1

very little increase at first . the membrane protein are stable at low temperatures
rapid rise from 50 * : cell membrane has been damaged at high temperatures with proteins denaturing
phospholipid bilaterally is damaged and fluidity is increased. so membrane is now personable and pigment leaks out
very little increase from 60* - most pigment has already seeped out

d) 1.
2. (membrane) proteins denature;
3. Increased fluidity / damage to the phospholipid bilayer;
Damage to (cell surface) membrane;

Question 2

controlled variables

Answer 2

-volume of water will affect the concentration of the solution and how much light is absorbed
-need to keep results for each temperature comparable

Question 3

What’s the effect of ethanol concentration

Answer 3

Increase leakage
Ethanol dissolves phospholipids creating gaps