cell fractionation

Question 1

why does the homogenate need to kept in an isotonic solution?

Answer 1

to prevent organelles suffering from plasmolysis or lysis as a result of osmotic gain or water loss to prevent the organelles from being damaged

Question 2

why does the homogenate need to kept in a buffered solution?

Answer 2

to maintain a constant pH to prevent enzymes from working and damaging the organelles

Question 3

what are the three conditions homogenates are placed in?

Answer 3

cold
isotonic
buffered

Question 4

why does the homogenate need to be kept in a cold solution?

Answer 4

reduces enzyme activity as it might break down the organelles

Question 5

what are the three steps to cell fractionation?

Answer 5

homogenisation
filtration
ultracentrifugation

Question 6

what is the purpose of cell fractionation?

Answer 6

allows scientists to study the functions of organelles
used to separate cell components

Question 7

what does it mean to homogenise?

Answer 7

to break up the tissue and break open the cells