Bordetella

Question 1

What is the cause of Bordetella pertussis?

Answer 1

Upper respiratory tract infection causing whooping cough

Question 2

Characteristics of bordetella pertussis

Answer 2

Encapsulated
strictly aerobic
oxidase positve
non fermentative
G - 
Nutritionally fastidious

Question 3

BP virulence factors

Answer 3

Adhesins:

• filamentous hemagglutinin
• pertussis toxin
• pili and pertactin

Toxins:

• pertussis toxin
• invasive adenylate cyclase
• dermonecrotic toxin
• tracheal cytotoxin - peptidoglycan fragments
• LPS - Lipid A and lipid X

Question 4

What type of cells produce mucus in the upper resp tract?

Answer 4

goblet cells

ciliated cells

Question 5

what is the pathogenesis of whooping cough

Answer 5

inhalation of aerosols –> bacteria adhere to ciliated epithelial cells – toxin production + accumulation –> damage to mucous cell or act on neurons and then paroxysmal couch ensues

Question 6

What leads to the invasive ACase?

Answer 6

S1 catalyzes ADP ribosylation rxn of Gi –> activates host ACase –> inc cAMP

Bacterial invasive ACase directly activates conversion of ATP to cAMP***

Question 7

What is an important clinical presentation of pertussis?

Answer 7

leukocytosis

Question 8

Pertussis diagnosis

Answer 8

Direct fluorescent Ab test
- fast, but not specific
PCR

Question 9

Treatment of Pertussis

Answer 9

Erythromycin

Question 10

Prevention of Pertussis

Answer 10

TdaP - new, DTP - old
new vaccines (acellular) not as efficacious as old vaccines (whole-cell)