BM: Enzyme-Controlled Reactions

Question 1

Describe an experiment that investigates the effects of temperature on an enzyme-controlled reaction:

Answer 1

1. Set boiling tubes with same vol + conc. of hydrogen peroxide
2. Add equal vol of buffer solution to keep pH constant
3. Put boiling tubes in water bath set to different temperatues.
4. Do the same to tubes containing catalase + leave 5 mins
5. Use pipette to add same vol + con catalase to each boiling tube.
6. Attach bung + delivery tube
7. Record how much oxygen is produced in 60s
8. Repeat at each temp x3 to find av.