BM: Enzyme Action Flashcards
What are enzymes?
Globular proteins that speed up chemical reactions by acting as biological catalysts.
What are catalysts?
Molecules that alter the rate of reaction without undergoing permanent changes themselves.
Can be reused repeatedly and are useful in small amounts.
What do enzymes catalyse?
At what levels do they do this?
Catalyse metabolic reactions - both at a cellular level (eg. respiration) and for the organism as a whole (eg. digestion in mammals).
What can enzymes affect?
Structures in an organism (eg. production of collagen) and functions (like respiration).
Enzyme action can be what?
Intracellular - within cells.
Extracellular - outside cells.
What type of molecule is an enzyme?
A globular protein.
What is the active site of an enzyme?
The part of the enzyme where the substrate molecules bind to.
It has a specific shape.
What is activation energy?
The initial amount of energy required to start a chemical reaction.
For a reaction to take place, what conditions must be satisfied?
- Molecules must collide with sufficient energy to alter the arrangement of their atoms.
- Free energy of products must be less than that of substrates.
- Activation energy must be overcame.
What is free energy?
The energy of a system that is available to perform work.
What is activation energy often provided as?
Heat
How do enzymes speed up the rate of reaction?
By lowering the activation energy of the reaction it catalyses.
What forms when a substrate fits into the active site of an enzyme?
An enzyme-substrate complex
What actually lowers the activation energy of the reaction?
What are the reasons why?
The formation of the enzyme-substrate complex.
- If the two substrate molecules are joining, attaching to an enzyme holds them closer together, reducing repulsion making them bond more easily - less energy is required to do this.
- If catalysing a breakdown reaction, fitting into an active site puts more strain on bonds in substrate, so they break up more easily - less energy is required to do this.
What was the early model of enzyme activity?
The lock and key model.