Blood Histo Flashcards

1
Q

what is the anitcoagulant typically used in a blood sample

A

EDTA

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2
Q

the ratio of RBC to the total volume of blood

A

hematocrit

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3
Q

what are the approximate normal levels of blood

A

55-plasma
45 RBC
1-buffy coat

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4
Q

what does the buffy coat consist of

A

leukocytes and platelets

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5
Q

what does CBC usually include

A

RBC count, Hb, Hct, WBC count, platelet count, and RBC indices

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6
Q

what is the pH of plasma

A

7.4

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7
Q

what does plasma consist of

A

albumins, globulins, fibrinogen

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8
Q

what are RBC typically stained with

A

Giesma or Wright (turn salmon)

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9
Q

what is the composition of a RBC membrane

A

40-lipid
10 carb
50 protein

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10
Q

caused by synthesis of of abnormal spectrin that exhibits defective binding to band 4.1 protein. produce fragile RBC

A

hereditary spherocytosis

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11
Q

when would a rhogam shot be needed

A

when a RH- mother delivers her fist RH+ baby

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12
Q

what is the mean corpuscluar volume

A

average volume of RBC

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13
Q

what is mean corpuscualr hemoglobin

A

average amount of Hb per RBC

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14
Q

how is MCHC determined

A

divide hemoglobin by hematocrit

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15
Q

what is polycythemia

A

increased concentration of RBC

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16
Q

what are the proinflammatory cytokines

A

TNF-alpha and IL-1

17
Q

which WBC contains histamine and leukotrienes

A

basophils

18
Q

which WBCs have receptors for IgE

A

basophils

19
Q

CD4 are what kind of cell

A

helper T cell

20
Q

CD8 are what kind of cell

A

cytotoxic T cell

21
Q

what are the two types of agranulocytes

A

leukocytes and monocytes

22
Q

what is the function of a monocyte

A

phagocytosis

23
Q

when do platelets become activated

A

when they come into contact with subendothelial collagen

24
Q

what releases con willebrand factors

A

injured endothelial cells

25
Q

what are the two pathways for coagulation

A

tissue factor pathway (extrinsic)(rapid) and contact activation pathway (intrinsic)(slow)

26
Q

These resemble large leukocytes

A

hemotopoietic stem cells