Biology Practicals

Question 1

In 6 Steps How You Prepare a Slide
(PRACTICAL)?

Answer 1

• 1) Add a Drop of Water to a Middle of a Clean Slide.
• 2) Cut Up a Onion and Take off 1 Layer.
• 3) Use Tweezers To Peel Of Some Epidermal tissue from the bottom of the layer.
• 4) Using The Tweezers Place the skin into the water on the slide.
• 5) add a drop of iodine Solution The Iodine Solution is a Stain and It Can Make It Easier to see.
• 6) Place a Cover Slip On Top.

Question 2

in 4 Steps How do you use the light microscope to look at the slides (PRACTICAL)?

Answer 2

• 1) Clip the Slide onto the Stage and Select The Objective Less With the Lowest Magnification.
• 2) Use The Coarse Adjustment Knob To Move the stage up to just below the Objective Lens.
• 3) Look Down the Eyepiece and move the stage downwards until the image is roughly in focus.
• 4) Move the fine Adjustment Knob Until you get a Clear Image.

Question 3

Name the 9 Steps Of Osmosis (PRACTICAL)?

Answer 3

• 1) First Cut Up a Potato Into Cylinders With The Same Length and Width.
• 2) then get 2 Beakers - 1 With Pure Water and Another with Concentrated Sugar Solution (E.g 1 Mol).
• 3) You Can Also Have a Few other Beakers With Less Concentrated Sugar Solution (E.g 0.2,0.4).
• 4) Measure The Mass Of Each Potato Cylinder and put 1 in each beaker and leave it for 24 hours.
• 5) Then Take Them Out and Dry Them With a Paper Towel and Measure Their Masses Again.
• 6) If The Mass Has Increased Water Has Moved Into The Potato and If Its Decreases water has Moved Out Of The Potato.
• 7) You Can Calculate the Percentage Change and Compare the Effects.

Question 4

Name 8 Steps Of Investigating Enzymes Reactions (PRACTICAL)

Answer 4

• 1) Put a Drop Of Iodine Solution Into Every well Of Spotting Tile and Set a Water Bath at 35°.
• 2) Add Some Amylase Solution and Buffer Solution With a PH Of 5 to a Boiling Tube.
• 3) Put The Boing Tubes in the Water Bath and Wait For 5 Minutes.
• 4) Add some starch solution to the boiling tube and mix and start a Stopwatch.
• 5) Every 30 Seconds take a Sample from the boing tube using a dropping pipette and Put a Drop of the sample into a well on the spotting tile.
• 6) When The Iodine Solution Stays browny-oranre all the starch in the sample has been Brocken down and recode how long it takes.
• 7) Repeat the experiment with buffer solutions of Different PH Values but as the PH Changes and the Time Taken for Starch to be Brocken down Will Also Change.

Question 5

Give 4 Steps Of Preparing Food Sample (PRACTICAL) Food Tests 1?

Answer 5

• 1) Get a Piece Of Food and Breck It Up Using a Pestle and Mortar.
• 2) Transfer The Ground up Food to a Becker and Add some Distilled Water.
• 3) Give The Mixture a Good Stir With a glass Rod to Dissolve some of the Food.
• 4)Filter The Solution Using a Funnel Lined Up with Filter Paper This Will Get Rid of The Solid Bits of Food.

Question 6

Name 5 Steps To Use The Benedicts Test to test for Sugars (PRACTICAL) Food Tests 2?

Answer 6

• 1) Prepare a Food Sample And Transfer 5 cm³ to a test tube.
• 2) Prepare a Water Bath at 75°.
• 3) Add Some Benedict Solution To The Test Tube About 10 Drops Using a Pipette.
• 4) Place The Test in the Water Bath Using a Test Tube Holder and Leave It for 5 Minutes.
• 5) the Solution In The Test Tube Will Change from The normal blue colour to green,Yellow or Brick Red.

Question 7

Name 3 Steps to Test For Starch (PRACTICAL) - Food Tests 3?

Answer 7

• 1) Make a Food Sample and Transfer 5 cm³ to a Test Tube.
• 2) Add a Few Drops Of Iodine Solution Gently Shake The Tube to Mix It Up.
• 3) If The Sample Contains Starch the Solution will Change From Brown-Orange to Black or Blue -Black.

Question 8

Name 3 Steps to Test For Proteins (PRACTICAL) - Food Tests 4?

Answer 8

• 1) Prepare a Sample of Your Food And Transfer 2 cm³ To a Test Tube.
• 2) Add 2 cm³ of Biuret Solution to the Sample Gently Shake It.
• 3) If The Food Sample Contains Protein The Solution will change from blue to Purple.

Question 9

Name 3 Steps to Test For Lipids (PRACTICAL) - Food Tests 5?

Answer 9

• 1) Prepare a Food Sample By Breaking The Food Using a Pestle and Mortar But Don’t Filter It Transfer 5 cm³ to a Test Tube.
• 2) Add 3 Drops of Sudan 3 Solution to the test tube and Gently Shake It.
• 3) If The Sample Contains Lipids the Mixture Will Separate out Into 2 Layers And The Top Layer will Be Bright Red.

Question 10

In 8 Steps Describe the Effect on Light Intensity (PRACTICAL)

Answer 10

• 1) Start by Taking a Boiling Tube and placing it 10 cm away from an LED Light Source.
• 2) Next fill the boiling tube with Sodium hydrogen carbonate solution.
• 3) Then put a piece of pondweed into the boiling tube with the cut end at the top.
• 4) Next Leave The pondweed for 5 Minutes to acclimate to the conditions of the boiling tube.
• 5) Then Bubbles of Oxygen Should be produced from the cut end of the pondweed.
• 6) Start a stopwatch and count how many bubbles are produced in 1 minute.
• 7) Then Repeat in 2 more times to calculate the mean.
• 8) And do the whole experiment again and move the boiling tube to 20 cm,30 cm and 40cm.

Question 11

In 6 Steps Investigate Reaction Time (PRACTICAL)?

Answer 11

• 1) The Person Begin Tested should sit with their arm resting on the edge of the table.
• 2) hold a ruler between their thumb and forefinger and Make sure the 0 end of the ruler is level with their thumb and finger.
• 3) Then let it go without giving any warning and the person should catch the ruler as quickly as they can.
• 4) Reaction time is Measured by the number on the ruler where it’s caught The Number should be Read from the Top of The Person Thumb.
• 5) Repeat the Test Several Times then Calculate the Mean Distance that the ruler fell.
• 6) Then give the person being tested a caffeinated drink and after 10 minutes repeat step 1 to 6.

Question 12

In 4 Steps how do you in the abundance of daisies using random sampling method (PRACTICAL)?

Answer 12

• 1) Collect a Quadrat (0.25 m²), Trundle Wheel and a Tape Measure.
• 2) Generate 20 Random Coordinates.
• 3) Drop The quadrat and count how many daises’ there is.
• 4) Use the trundle wheel to calculate the school field area.

Question 13

In 4 Steps how do you set up a transect line and how do you collect data (PRACTICAL)?

Answer 13

• 1) Collect a Transect up to 10 metres.
• 2) Then put the start of the transects where there is lots of light and the other point where their is less light using a light meter.
• 3) Then you need a quadrat and you place it on the 1 meter.
• 4) You have to use the light meter to count see where there is a lot of light and how many daises are growing in that area.