Biology Practicals Flashcards
1
Q
In 6 Steps How You Prepare a Slide
(PRACTICAL)?
A
- 1) Add a Drop of Water to a Middle of a Clean Slide.
- 2) Cut Up a Onion and Take off 1 Layer.
- 3) Use Tweezers To Peel Of Some Epidermal tissue from the bottom of the layer.
- 4) Using The Tweezers Place the skin into the water on the slide.
- 5) add a drop of iodine Solution The Iodine Solution is a Stain and It Can Make It Easier to see.
- 6) Place a Cover Slip On Top.
2
Q
in 4 Steps How do you use the light microscope to look at the slides (PRACTICAL)?
A
- 1) Clip the Slide onto the Stage and Select The Objective Less With the Lowest Magnification.
- 2) Use The Coarse Adjustment Knob To Move the stage up to just below the Objective Lens.
- 3) Look Down the Eyepiece and move the stage downwards until the image is roughly in focus.
- 4) Move the fine Adjustment Knob Until you get a Clear Image.
3
Q
Name the 9 Steps Of Osmosis (PRACTICAL)?
A
- 1) First Cut Up a Potato Into Cylinders With The Same Length and Width.
- 2) then get 2 Beakers - 1 With Pure Water and Another with Concentrated Sugar Solution (E.g 1 Mol).
- 3) You Can Also Have a Few other Beakers With Less Concentrated Sugar Solution (E.g 0.2,0.4).
- 4) Measure The Mass Of Each Potato Cylinder and put 1 in each beaker and leave it for 24 hours.
- 5) Then Take Them Out and Dry Them With a Paper Towel and Measure Their Masses Again.
- 6) If The Mass Has Increased Water Has Moved Into The Potato and If Its Decreases water has Moved Out Of The Potato.
- 7) You Can Calculate the Percentage Change and Compare the Effects.
4
Q
Name 8 Steps Of Investigating Enzymes Reactions (PRACTICAL)
A
- 1) Put a Drop Of Iodine Solution Into Every well Of Spotting Tile and Set a Water Bath at 35°.
- 2) Add Some Amylase Solution and Buffer Solution With a PH Of 5 to a Boiling Tube.
- 3) Put The Boing Tubes in the Water Bath and Wait For 5 Minutes.
- 4) Add some starch solution to the boiling tube and mix and start a Stopwatch.
- 5) Every 30 Seconds take a Sample from the boing tube using a dropping pipette and Put a Drop of the sample into a well on the spotting tile.
- 6) When The Iodine Solution Stays browny-oranre all the starch in the sample has been Brocken down and recode how long it takes.
- 7) Repeat the experiment with buffer solutions of Different PH Values but as the PH Changes and the Time Taken for Starch to be Brocken down Will Also Change.
5
Q
Give 4 Steps Of Preparing Food Sample (PRACTICAL) Food Tests 1?
A
- 1) Get a Piece Of Food and Breck It Up Using a Pestle and Mortar.
- 2) Transfer The Ground up Food to a Becker and Add some Distilled Water.
- 3) Give The Mixture a Good Stir With a glass Rod to Dissolve some of the Food.
- 4)Filter The Solution Using a Funnel Lined Up with Filter Paper This Will Get Rid of The Solid Bits of Food.
6
Q
In 4 Steps To Use The Benedict’s Test to test for Sugars (PRACTICAL) Food Tests 2?
A
- 1) Prepare your food sample, transfer 5cm³ of that sample to the test tube and prepare a water bath for 75°.
- 2) When your water bath is ready, add 10 Drops of benedict solution to your test tube and place the test tube in the water bath for 5 mins.
- 3) For safety use a test tube holder and place it in the water bath and point the tube away from you and anybody else.
- 4) If reducing sugars is present the solution should stay blue, But if reducing sugars isn’t present the solution should change to green, yellow and brick red depending on concertation.
7
Q
Name 2 Steps to Test For Starch (PRACTICAL) - Food Tests 3?
A
- 1) Take 5cm³ of your food sample, add a few drops of iodine solution.
- 2) The solution will turn brownie - orange colour, next give it a gentle shake and if the sample contains starch the solution should change brownie organa to blue-black colour.
8
Q
Name 2 Steps to Test For Proteins (PRACTICAL) - Food Tests 4?
A
- 1) Add 2cm³ of your sample to a test tube and then add another 2cm³ of your biuret solution to your sample.
-2) It should turn blue, Then give it a gentle shake and if your food ample contains protein it will change from blue to pink or purple.
9
Q
Name 3 Steps to Test For Lipids (PRACTICAL) - Food Tests 5?
A
- 1) Prepare a Food Sample By Breaking The Food Using a Pestle and Mortar But Don’t Filter It Transfer 5 cm³ to a Test Tube.
- 2) Add 3 Drops of Sudan 3 Solution to the test tube and Gently Shake It.
- 3) If The Sample Contains Lipids the Mixture Will Separate out Into 2 Layers And The Top Layer will Be Bright Red.
10
Q
In 8 Steps Describe the Effect on Light Intensity (PRACTICAL)
A
- 1) Start by Taking a Boiling Tube and placing it 10 cm away from an LED Light Source.
- 2) Next fill the boiling tube with Sodium hydrogen carbonate solution.
- 3) Then put a piece of pondweed into the boiling tube with the cut end at the top.
- 4) Next Leave The pondweed for 5 Minutes to acclimate to the conditions of the boiling tube.
- 5) Then Bubbles of Oxygen Should be produced from the cut end of the pondweed.
- 6) Start a stopwatch and count how many bubbles are produced in 1 minute.
- 7) Then Repeat in 2 more times to calculate the mean.
- 8) And do the whole experiment again and move the boiling tube to 20 cm,30 cm and 40cm.
11
Q
In 4 Steps how do we Investigate Reaction times (PRACTICAL)?
A
- 1) Hold ruler between the persons thumb and index finger, Line it up with the 0cm mark, Drop without warning and Record distance before caught.
- 2) Calculate the Reaction time Using newtons equation of motion.
- 3) Repeat the Investigation again and Generate an Average. But the person will get better over multiple tries.
- 4) Lastly Add an Independent variable, Give the person A Caffeine Drink to see how that changes the reaction time.
12
Q
In 4 Steps how do you Measure the abundance of daisies using random sampling method (PRACTICAL)?
A
- 1) Collect a Quadrat (0.25 m²), Trundle Wheel and a Tape Measure.
- 2) Generate 20 Random Coordinates.
- 3) Drop The quadrat and count how many daises’ there is.
- 4) Use the trundle wheel to calculate the school field area.
13
Q
In 4 Steps how do you set up a transect line and how do you collect data (PRACTICAL)?
A
- 1) Collect a Transect up to 10 metres.
- 2) Then put the start of the transects where there is lots of light and the other point where their is less light using a light meter.
- 3) Then you need a quadrat and you place it on the 1 meter.
- 4)use the light meter to see where there is a lot of light and how many daises are growing in that area.
14
Q
Give 4 Steps Of Preparing Food Sample (PRACTICAL) Food Tests 1?
A
- 1) Get a Piece Of Food and Breck It Up Using a Pestle and Mortar.
- 2) Transfer The Ground up Food to a Becker and Add some Distilled Water.
- 3) Give The Mixture a Good Stir With a glass Rod to Dissolve some of the Food.
- 4)Filter The Solution Using a Funnel Lined Up with Filter Paper This Will Get Rid of The Solid Bits of Food.
