BIOL 321 Lab Part II Flashcards
Lophotrochozoa tree
Bilateria - Protostomia - Lophotrochozoa - 2 branches
1- Mollusca s.g. to Nemertea and Annelida
2. Bryozoa s.g. to Rotifer and Platyhelminthes
Nemertea
previously proboscis/ribbon worm
soft-bodied, non-segmented, slight torso-ventral flattening, ciliated ventral surface, abundant mucous glands, apparent lack of secondary body cavity but coelomate
Nemertea are coelomates?
lateral blood vessels are shrunk down coelomic compartments
epithelial lining derived from mesoderm, face lumen of blood vessel
Nemertean digestion
tubular, one-way digestive tract with mouth and anus
Nemertea characteristics
1-way digestive tract
eucoelomic compartments- circulatory system
eversible proboscis
proboscis
eversible, enclosed in proboscis sac (rhynchocoel), dorsal to anterior part of gut
shot out of body through pore dorsal to mouth
wall of sac is mesodermally-derived, and wrapped in muscle
Nemertean habitat
mainly marine
few fresh, few terrestrial (low resistance to desiccation)
under rocks, burrow in sediment
Nemertean species
ca. 1200
Tubulanus polymorphus
local nemertean
can be over 1m long
thin, long body, easily torn
vivid orange color, conspicuous coloration = chemical
ejection of proboscis
circular muscles contract - fluid pressure in sac increases - proboscis shoots out - turns inside out
proboscis elaborations
some have calcified stylet and secrete neurotoxin
how does proboscis return to rhynchocoel
proboscis retractor muscle
Nemertea movement
mucco-ciliary gliding
muscular wave crawling and dorsoventral swimming
Lophophore
organ for capturing suspended food
circular/horseshoe-shaped fold of body wall that encircles mouth and bears ciliated tentacles
Lophophorates
former phylum that contained Bryozoans, Brachiopods, and Phoronids that all contain lophophore
convergence
Bryozoa habitat
marine, freshwater
attached to rocks, plants, animals, docks
marine Bryozoans
lophophore forms circle of tentacles with mouth in centre
Bryozoan characteristics
u-shaped digestive tract, anus outside lophophore
almost all colonial
various growth/body forms
Bryozoan body forms
erect, branching, flat, encrusting
individual bryozoan
zooids
freshwater bryozoans
horseshoe shaped lophophore
zooids secrete
zooecium exoskeleton
Bryozoan reproduction
sexual, asexual (budding)
zooecium material
chitinous
proteinaceous
calcareous
Membranipora
genus of marine bryozoan
flat, encrusting colonies, often on kelp
Bugula
marine bryozoan
erect, branching colonies
lophophore retracted into zooecium
with lophophoral retractor muscles
Bryozoan feeding
beat ciliary tracts on lophophore tentacles - water currents - bring in suspended particle - cilia capture particle - transfer down tentacle to mouth
formation of new Bryozoan colony
settlement and metamorphosis of a ciliated larva = original zooid of the colony
Membranipora Bryozoan colony
ancestor zooid metamorphoses to form double zooid = ancestrula
individuals of a colony are identical, no polymorphism
frontal membrane not calcified
Bryozoan polymorphism
autozooids - feeding, produce gametes
heterozooids - defense zooids
Bugle heterozooid
avicularium
non-feeding, look like bird beak, open and close jaw - prevent settling of microorganisms on them
lower beak of avicularium
mandible
homologous to operculum of autozooid
Membranipora energy use
no frontal membrane calcification
divert energy to rapid growth, differentiation, and sexual maturation b/c seasonal species
freshwater bryozoan class
all one class larger zooids, more lophophoral tentacles in horseshoe shape produce overwintering bodies
overwintering bryozoan body
statoblast - yolky mesothelial cells congregate - cohesive mass - surrounded by epithelium - secret chitinous covering - covering may have elaborate looks or air pockets
floating, freezing, desiccation protection
germinate in spring when conditions less harsh
Mollusca phylogeny
Aculifera s.g. Conchifera
Aculifera – Aplacophora, Polyplacophora
Conchifera – (Monoplacophora, Cephalopoda) s.g. (Scaphopoda (Bivalvia, Gastropoda))
Gastropoda phylogeny
Vetigastropoda s.g. Patellogastropoda
Neritimorpha s.g. Caenogastropoda s.g. Heterobranchia
Mollusca species
ca. 80,000
second only to arthropods (in Metazoa)
Mollusc basic body plan
2 regions: cephalopodium (head + muscular foot), visceropallium (visceral organs, mantle)
mantle
pallium
dorsal covering of epidermal epithelium
secretes calcareous exoskeleton (1+ hard shell pieces) or spicules
mantle cavity
where mantle epithelium folds inward around periphery of foot and delineates a pocket – open to surrounding water
In mantle cavity
Pallial organs: 1+ gills = ctenidia, 1-2 sensory organs = osphradia (s. um)
anus, nephridiopore, gonoduct
tongue-like mollusc structure
radula
ribbon of transverse rows of teeth repetitively protruded from mouth
-lacking in bivalves, secondarily lost in some gastropods
Mollusca sizes
1mm (snail) - 18m (giant squid)
gastropod larva
veliger
Polyplacophora
‘many shells’
chitons
live on hard substrate, intertidal-shallow subtidal
dorso-ventrally flattened
Polyplacophora movement
slow crawl with ventral, muscular foot
polyplacophora feeding
radula scrape algae, bacteria off rock surface
distinctive chiton feature
8 overlapping, articulating dorsal plates embedded in mantle tissue
plates
valves
chiton mantle tissue surrounding valves
girdle
chiton cephalization
minimal - sedentary lifestyle
chiton anus
pigmented orange-brown strips in roof of mantle cavity on either side of anus = osphradia
chiton osphradia
concentration of sensory cells
posterior sensory cells??? (unknown why)
molluscan gills with filaments on both sides of the central axis
bipectinate ctenidia
how chitons cling so well
grip hard surface with peripheral edge of muscular girdle - elevate roof of mantle cavity - create suction
water flow through mantle cavity of chiton
enters pallial groove – down pallial groove, through gill chambers – exits pallial groove at anus
smooth area of chiton valve embedded in girdle tissue
articulamentum - hard, white, CaCO3
rough, exposed area of chiton valve
tegumentum - brown, rough, mostly conchiolin protein
canal cells in chiton valves
canals run through articulamentum in to tegumentum, house sensory cells (aesthetes)
some are photoreceptors
chiton muscle groups for flexing shell valves
rectus muscles - slender bundles of longitudinal muscle extend down side of midline
transverse muscle - thick muscle pads at junction between shell valves. from dorsal side of one articulamentum to ventral side of next most articulamentum
chiton salivary glands
branched, yellow, open into buccal cavity
secrete mucus to lubricate radula
esophageal glands
large, paired, ‘sugar glands’, secrete amylase to initiate food digestion
Chiton gonads
dioecious = gonochoristic = either testis or ovary not both
Radular teeth mounted on
basal strip of chitin
radular teeth deep in radular sac
small, incompletely formed, translucent
new teeth continuously secreted at distal end of radular sac - move forward in conveyor belt fashion
new teeth secreted by
odontoblasts
why are new teeth continuously secreted
continuously worn/abraded off, need to be replaced
radular teeth organization
transverse rows of 17 teeth - central tooth + 8 lateral on each side of central
1 lateral on each side is larger and black = tricuspid
why is radula tricuspid black
magnetite cap
tissue beneath radula
2 cartilage-like rods = odontophoral cartilages
protracted from mouth with radular ribbon to press teeth firmly against substrate
odontophoral cartilage + radular ribbon
buccal mass
buccal mass muscles
myriad muscles
execute feeding motion, red (contain myoglobin) - supply hard working muscles w/ O2
characters of chitons shared with other molluscs
CaCO3 shells secreted by mantle tissue ribbon of radular teeth mantle cavity containing ctenidia, osphradia, and receives anus, nephridiopores, gonopores bipectinate ctenidia muscular foot on ventral side
bipectinate
branched like a feather on both sides of a main shaft
distinctive characters of chitons
8 overlapping valves - change body shape for unflat surface sugar glands magnetite capped teeth straight digestive tract can suction body to rock
Class Gastropoda
largest class of molluscs in species and diversity
Gastropoda distinctive character
torsion
torsion
brings anus, mantle, pallial organs to anterior position over back of head
180º rotation of visceropallium relative to cephalopodium
gastropod shell coil
asymmetrically around central axis
gastropod shell central axis
columella
aids in protection shell provides gastropod
operculum - hardened plate of protein, secreted by back of foot, seals aperture shut
Vetigastropoda
keyhole limpet
vetigastropoda characteristics
bipectinate ctenidia, perforated shell, 2 ctenidia, 2 osphradia, 2 hypobranchial glands
broadcast spawn, external fertilization
Caenogastropoda
dog whelk
monopectinate ctenidium, gill axis fused along length to roof of mantle cavity, shells not perforated, 1 ctenidium, 1 osphradium, 1 hypobranchial gland
internal fertilization, eggs deposited in benthic egg capsules, veliger larvae
monopectinate ctenidium
gill filaments arise from only one side of central gill axis
Heterobranchia
sea slugs, pond snails
Heterobranchia characteristics
reduction/loss of calcified shell, minimal torsion, true ctenidium often absent replaced by gas exchange structures
internal fertilization, eggs deposited in benthic egg capsules, veliger larvae
torsion in vetigastropods
full extent of torsion
mantle cavity fully anterior
anus mid-dorsally over back of head
torsion in caenogastropods
anterior mantle cavity
anterior anus towards right side rather than mid-dorsal
heterobranchia torsion
little evidence of torsion in adult stage
importance of directing water into mantle cavity
oxygen over ctenidia filaments
flush feces and urine out of mantle cavity
Heterobranch ctenidia
lost altogether
Gastropod perforation gas exchange
vetigastropods - 1+ shell perforations, 2 ctenidia on either side of anus
water enters mantle cavity on both sides of head, flows between filaments, exits mantle cavity by passing out of shell perforation
Caenogastropod gas exchange strategy
1 ctenidium and osphradium located on left side of anterior mantle cavity
anus toward right side
water enters left side - passes over osphradium - flows between ctenidia filaments - out right side of mantle cavity picking up waste
osphradium
patch of sensory epithelium
may monitor possible contaminants or silt levels
Heterobranchia gas exchange
many lack ctenidium, mantle cavity may be lacking in adult, replaced ctenidium with various other gas exchange structures - anal branchiae, cerata
Pulmonates
mostly terrestrial and freshwater heterobranchs
usually breathe atmospheric O2
mantle cavity almost entirely sealed-off
pulmonate mantle cavity opening
pneumostome
pulmonate internalized mantle cavity
lung - muscles pump air in and out via pneumostome
gastropod shell mineral
CaCO3
calcite or aragonite
keyhole-limpet shell
well-developed, heavily calcified, secondarily lost coiling, very wide aperture, no operculum
Nudibranch shell
only present in larval stage
adult defense by defensive chemical
mollusc larva
veliger- planktonic, swim, some feed, coiled shell, foot, operculum, velum
velum
two lobes extending from either side of head
2 tracts of cilia around peripheral edge of each lobe
prototroch + metatroch
veliger prototroch
long cilia power swimming and bring phytoplankton toward veliger
Veliger effective stroke
prototrochal cilia down
veliger metatroch
runs parallel to prototroch, shorter cilia, power stroke is upward toward prototroch
veliger feeding
food particles caught between 2 ciliary bands and collected within food groove (also ciliated) - carry food particles to mid-ventral mouth
gill filaments arising from only one side of central axis of ctenidium
monopectinate
dorsal lateral outgrowths on the anterior surfaces of nudibranchs
cerata
cerata function
anal branchiae
aid in resperation
also attach and defense
nudibranch dorsal outgrowths (some nudibranchs)
cnidosacs
central axis of gastropod shell
columella
Class Bivalve phylogeny
Protobranchia
Autobranchia - Pteriomorpha, Heteroconchia
Pteriomorpha includes
mussels, scallops, oysters
Heteroconchia includes
all other bivalves
bivalves are primarily adapted for
life buried in sediment
bivalve burial adaptations
laterally flattened foot
bi-valve shell, hinged dorsally
sensory structures
bivalve foot adaptation
thrusting into sand/mud during burrowing
bivalve shell adaptation
enclose to protect soft tissues from abrasive sediment, prevent collapse of
bivalve sensory structure adaptation
concentrated along periphery of mantle fold bordering edge of shell valves rather than on head
why are bivalve sensory structures around valve periphery and not on head
head lies deep within spaced enclosed by shell valves
Protobranchia characteristics
deposit feeders, single pr. bipectinate ctenidia, short gill filaments for gas exchange only, taxodont hinge dentition
protobranchia deposit feeding
use tentacle-like feeding appendages extending from either side of mouth - collect, deliver particles to mouth
protobranchia feeding appendages
palp proboscis
vast majority of extant bivalves
Autobranchia
vast majority of Autobranchia have
lamellibranch ctenidia
lamellibranch ctenidia
greatly elongated ctenidia filaments, arising from either side of central axis, elongated to extent that filaments on each side fold back on themselves
how is water drawn in to mantle cavity of most molluscs
beating of lateral cilia of ctenidia filaments
lamellibranch ctenidia function
used for gas exchange and suspension feeding - phytoplankton captured, delivered to mouth by cooperative activities of specialized ciliary tracts
Autobranchia hinge dentition
heterodont
Pteriomorphia characteristics
epibenthic, anchor to sediments/rocks w/ byssal threads or directly cement shell valve to substrate
much reduced foot
mantle margins not fused
why do pteriomorphs have reduced foot
absence of burrowing activity (also why mantle margins not fused)
most extant bivalves are in which auto branch clade
Heteroconchia
2 sections of lamellibranch ctenidium
demibranchs
demibranchs formed by
long row of folded gill filaments arising from either side of gill axis
byssal thread characteristics
proteinaceous, secreted by gland at base of foot, present in most bivalve juveniles, mussels retain gland while most bivalves lose as adults
bivalve shells
outer periostracum (tough protein)
underlying biomineral
periphery corrugated to form 3 lobes
how bivalve shells are enlarged
secretion of periostracum and bxomineral by the peripheral edge of each lateral mantle fold
bivalve shells, outer lobe
secretes periostracum and biomineral
bivalve shell, middle lobe
sensory structures
row of light-reflecting eyespots
bivalve shell, inner lobe
muscular
pallial muscles from lobe to inner wall of shell
forms broad flap projecting into gape of shell
Pteriomorph eye
only bivalves with differentiated eye structures w/ lens, retina, pigment cells
bivalve shells are held together by
hinge ligament + adductor muscles + pallial muscles
adductor muscles
anchored on inner valve surface
extend between 2 valves
pull valves closed
most bivalves have 2 (A&P), same size
Mussel adductor muscles
anterior smaller than posterior