biochem pre lab 3 Flashcards
what the fuck
Protein comes from the Greek word
Proteios which “means first of importance.”
It is considered a protein when it has at least 1___ amino acid residues. Otherwise, it is known as a 2__________
1 50, 2 Peptide
Proteins assume a three-dimensional structure known as their ____________
native conformation
________ refers to the disruption and unfolding of proteins, thus losing their biological activity
Denaturing
For example, when a white solid from an egg converts from transparent liquid to white solid clumps, it is called __________.
coagulation
_______ _______ are generally defined as metals with relatively high densities, atomic weight, or atomic number
Heavy metals
______ ____ is an acid derived from one or more inorganic compounds.
Mineral acid
2 tests that help in the precipitation of proteins by strong mineral acid
Heller’s test
Robert’s test
________ ______ are high molecular weight anions
alkaloidal reagents
three procedures for the precipitation of protein
precipitation of protein by strong mineral acid
precipitation of protein by Heavy Metal Salts
precipitation of protein by alkaloidal reagents
_______ is the sticking of molecules together to form a solid that will come out of the solution
Precipitation
A ______ _______ is a chemical reaction that is used to transform colorless chemical compounds into colored derivatives which can be detected visually
Color reaction
The 1______ Test, also known as 2________ test, is a general test for the presence of proteins which is dependent on the presence of peptide bonds
Buiret Test, Piotrowskis test
A positive result for the Buiret Test or Piotrowskis test
Violet color, if it contains peptides, proteoses, and peptones, a minimum of 2 peptide bonds
_______ test is a general test for the presence of proteins, which is dependent on the presence of free alpha amino groups.
Ninhydrin test
Ninhydrin test:
Free alpha-amino groups of proteins and amino acids undergo an ________ _________ with ninhydrin
oxidative determinant
A positive result for the Ninhydrin test
Presence of diketohydrin, a color complex that is also known as Ruhemans purple. (Deep blue or violet color)
The __________ test is used to indicate the presence of amino acids containing phenyl (benzene ring), namely phenylalanine, tyrosine, and tryptophan
Xanthropethic test
Xanthropethic test:
The rings phenylalanine, tyrosine, and tryptophan undergo 1_______ with treatment with 2________ _____ _____ when heated
1 Nitration,2 concentrated nitric acid
A positive result for the Xanthropethic test
(Yellow to orange color)
________ reaction is a specific test for the presence of the amino acid tryptophan
Adamkiewicz reaction
A positive result for the Adamkiewicz reaction
Formation of violet ring
Adamkiewicz test:
The ______ group reacts with the glyoxylic acid in the presence of concentrated sulfuric acid
indole
______ ______test is a specific test for the presence of amino acid cysteine.
Reduced sulfur test
A positive result for the Reduced sulfur test
Lead sulfide (black precipitate.)
______ proteins contain only amino acid components
simple proteins
1________ proteins contain amino acids and non-amino acids called 2______ groups
1 conjugated, 2 prosthetic
___________ test is a general test for the presence of carbohydrates in a solution
molisch’s test
molisch’s test:
A protein with a carbohydrate prosthetic group is known as ________
Glycoproteins
A positive result for the molisch’s test test
Violet ring
________ ________ test is a test for finding a phosphate moiety.
Modified Numans
Modified Numans:
A conjugated protein containing a phosphate group is called a ____________
phosphoprotein
A positive result for the Modified Numans test
A canary yellow precipitate of ammonium phosphomolybdate
