BGM1002/L18 Translation I Flashcards
Give the 4 main features of the genetic code?
Triplet
Non-overlapping
Degenerate/redundant
Universal
How many combinations does a triplet code allow?
4^3
64
How many stop codons are there?
3
How many codons specify amino acids?
61
How many start codons are there?
1 (MET)
In what organism is the CUG codon reassigned from Leu to Ser?
Candida albicans (yeast)
How many species of tRNA exist in E. coli?
46
Describe the primary structure of tRNA. (3)
Small - 70-90 nucleotides
Have 5’ monophosphate (rather than 5’ triphosphate)
Contain modified bases
Name the 4 modified bases that exist in tRNA.
Ribothymidine
Pseudouridine
Dihydrouridine
Inosine
Describe the secondary structure of tRNA. (5)
D-loop (contains dihydrouridine)
T-loop (contains pseudouridine)
Variable arm
Anti-codon loop
Amino acid acceptor site
What is the 3’ terminal nucleotide sequence in tRNA?
-CCA
What are tRNAs joined to amino acids called?
Aminoacyl tRNA
Charged tRNA
What type of enzymes catalyse aminoacylation of tRNAs?
tRNA synthetases
Describe aminoacylation of tRNA. (3)
AMP added to carboxyl group of amino acid
High energy intermediate formed - aminoacyl adenylate
Aminoacyl adenylate reacts with appropriate uncharged tRNA
Which hydroxyl are amino acids joined to on tRNA?
2’ or 3’
Once joined, two species can interconvert