BGM1002/L02 Protein Structure Flashcards
What are proteins?
Complex polymers made of amino acid monomers joined by peptide bonds
How many levels of complexity do proteins exhibit?
4
What type of isomer are amino acids (in proteins)?
L-isomers
How many covalent bonds can carbon form?
4
What formation do electron shells form with 4 bonds in carbon?
sp3 hybridisation
How many possible configurations does carbon have with 4 different substituent groups?
2 stereoisomers
What do chiral enantiomers affect? And how?
Polarised light
Rotation of plane of light clockwise (+) or anticlockwise (-)
How is a chiral enantiomer indicated?
L-/D- or S/R
What does L-/D- mean?
Configuration relative to D-glyceradehyde
What does S/R mean?
Absolute configuration
How is absolute configuration determined?
Based on priority of substituent groups around chiral centre according to Cahn-Ingold-Prelog sequence
Give 2 examples of where D-amino acids are found in nature.
Bacterial cell walls
Non-ribosomal peptides
What handedness do alpha-helices have?
Right-handedness
How are interactions between proteins and other molecules specific?
They display a preference for specific chiral forms
What 3 uses does Thalidomide have?
Anti-cancer
Leprosy treatment
Graft-versus host disease treatment
What effect did Thalidomide have when given as anti-nausea to pregnant women?
Development arrest of limbs in foetuses
Which enantiomer of Thalidomide causes complications?
S-enantiomer
Which enantiomer of Thalidomide is therapeutic?
R-enantiomer
Why is there no safe way to administer R-Thalidomide?
Body converts R- to S-Thalidomide
What charge do acidic amino acids have?
Negative
What charge do basic amino acids have?
Positive
What is minimised when a protein folds?
Hydrophobic residue on its surface
What amino acid do most proteins start with?
Methionine
What type of bonding can uncharged side chains be involved with?
Hydrogen bonding