BACTERIOLOGY Flashcards
Smallest units capable of independent existence
Living Cells
Usually less than 5um in length
Prokaryotic Cell
Membrane-bound organelles are present
Eukaryotic Cells
80S ribosomes in cytoplasm; 70S ribosomes in mitochondria and chloroplasts
Eukaryotic Cell
Nucleic acid occurs as a single molecule, often circular
Prokaryotic Cell
Nuclear membrane and nucleolus absent
Prokaryotic Cell
Unicellular and are smaller and less complex than eukaryotic cells such as mammalian red blood cells
Bacteria
Bacteria occur as _______, _______, _________ forms and occasionally as ____________.
rods, cocci, helical ; branching filaments
Readily seen using conventional light microscopy
Red Blood Cell
Rod shaped cells, usually stained by the Gram method. Using bright-field microscopy, a magnification of 1000x is required to observe most bacterial cells.
Bacillus
Spherical-shaped cells, often occuring in chains or in grape -like clusters.
Coccus
Thin, helical bacteria, dark field microscopy (without staining) or special staining methods are required to demonstrate these unusual microorganisms.
Spirochaete
Yeasts, molds and mushrooms belong to a large group of non-photosynthetic eukaryotes.
Fungi
Produce filamentous microscopic structures called molds.
Multicellular fungi
Yeast; spherical or ovoid shape and multiply by budding
Unicellular fungi
Often occur in chains or grape-like clusters
Coccus
Reproduce by budding
Yeast
Branched structures (hyphae) composed of many cells.
Mould
Considered plant-like because they contain chlorophyll; free living in water, others grow on the surfaces of rocks and on other structures in the environment.
Algae
They are not cells and consist of nucleic acid, either DNA or RNA, enclosed in a protein coat called capsid.
Viruses
Viruses which invade bacterial cells.
Bacteriophages
Readily seen at magnification of 1000x
Coccus
devoid of nucleic acid, composed of abnormally folded protein capable of inducing conformational chages in host cell protein then damages long-lived cells such as neurons.
Prions
exhibit remarkable resistance to physical and chemical inactivation procedures.
Prions
Stain of Gram-positive bacteria
purple-blue
Stain of Gram-negative bacteria
red
Gram positive, cluster-forming cocci; nonmotile, facultative anaerobes, catalase-positive; fermentative, grow on non-enriched media.
Staphylococcus species
detects the presence of a bound coagulase or clumping factor on the bacterial surface.
Slide test
detects free coagulase or staphylocoagulase which is secreted by the bacteria into the plasma.
Tube test
Staphylococcus aureus virulence factors
coagulase
protein A
leukocidin
beta and alpha toxin
toxic-shock syndrome toxin (TSST)
Toxins produced by S. aureus
Alpha hemolysin
Beta hemolysin
Gamma hemolysin
Delta hemolysin
Leukocidin
Enterotoxin
Enzymes produced by S. aureus
Coagulase (free)
Hyaluronidase (spreading factor)
Deoxyribonuclease
Fibrinolysin (staphylokinase)
Lipase
Protease
Produces toxins similar (but not identical) to S. aureus
S. hyicus
Induced recurrent pyoderma- mast cell degranulation. Results in an intense inflammatory reaction– leads to pruritus, erythem, etc.
S. intermedius
Sucklers and weaned pigs up to 3 months. Widespread excessive sebaceous secretion, exfoliation and exudation on the skin surface
Exudative epidermitis
Ixodes ricinus is a vector for the rickettsial agent of tick-borne fever.
Tick pyemia
A chronic, suppurative granulomatous condition. It can occur within a few weeks of castration in the horse due to infection of the stump of the spermatic cord (scirrhous cord).
Botryomycosis
Gram positive cocci in chains; facultative anaerobes, usually motile, catalase negative; fastidious, requiring enriched media; form small, usually hemolytic, transclucent colonies.
Streptococci
Classified based on type of hemolysis, biochemical tests and by serological means (Lancefield groups) using precipitation test based on the cell surface carbohydrate moiety.
Streptococci
complete hemolysis indicated by clear zones around colonies
Beta-hemolysis
partial or incomplete haemolysis indicated by greenish or hazy zones around colonies
Alpha-haemolysis
denotes no observable changes in the blood agar around colonies
Gamma-haemolysis
elaborates a factor which completely lyses the red cells already damaged by the beta hemolysin of staphylococcusu aureus, producing a characteristic clear ‘arrow head’ pattern of complete hemolysis.
Streptococcus agalactiae
highly selective for the streptococci and also indicates aesculin hydrolysis and the type of haemolysis.
Edwards medium
causes strangles, highly contagious, severe purulent infection of the upper respiratory tract and draining lymph nodes of solipeds
Streptococcus equi
Common finding of strangles
Guttural pouch empyema
considered to be an immune-mediated disease, may occur in some affected horses 1 to 3 weeks after initial illness.
Purpura Haemorrhagica
Virulence factors of streptococcus equi
- Cytotoxin
- M protein
- Hyaluronic Acid Capsule
causes ruminant mastitis, neonatal sepsis and meningitis in humans
Streptococcus agalactiae
GBS virulence factors
- Cell surface structures
- Major exotoxin
- CAMP Factor
- Neuraminidase
- Hemolysin
- Vasoactive extracellular toxin
- Hyaluronidase
carried in the mouth, skin lesions of the udder, and in vagina
Streptococcus dysgalactiae
commensal of bovine GIT & skin, causes opportunistic disease (primarily mastitis) in cows reared under poor management conditions.
Streptococcus uberis
carrier pigs harbor the organism in tonsillar tissues; causes meningitis, arthritis, septicemia and bronchopneumonia in pigs of all ages, and with sporadic cases of endocarditis, neonatal deaths and abortion.
Streptococcus suis
causative agent of “swine strangles”/jowl abscessation, a severe cervical/mandibular lymphadenopathy.
Streptococcus porcinus
causes disease in several host species.
Streptococcus zooepidemicus
Pleomorphic, gram-positive bacteria which occur in coccoid, club and rod forms (coryneform morphology)
Genus corynebacterium
Caused by the non-nitrate reducing biotype of C. psedutuberculosis.
Caseous lymphadenitis
Caused by the non-nitrate reducing biotype of C. psedutuberculosis.
Caseous lymphadenitis
Caused by the non-nitrate reducing biotype of C. pseudotuberculosis.
Caseous lymphadenitis
Sporadic cases caused by the nitrate reducing biotype of C. pseudotuberculosis.
Ulcerative lymphangitis in horses and cattle
Characterized by ulceration around the preputial orifice, with a brownish crust developing over the lesion. Common in merino sheep and angora goats, caused by C. renale.
Ulcerative (enzootic) balanoposthitis (pizzle rot)
Organisms belonging to the C. renale group can be isolated from the vulva, vagina and prepuce of apparently normal cattle.
Bovine pyelonephritis
Virulence factor of Bovine pyelonephritis
Pili/fimbriae
Their ability to break down urea into ammonia
Produce cell lysis agent called Renalin
Gram-positive rods or cocci; Growth on non-enriched media; Aerobic, non-motile
Genus Rhodococcus
Salmon-pink mucoid colonies with no haemolysis; Soil saprophyte
Genus Rhodococcus
Produces a factor which completely lyses the red cells previously damaged by the beta haemolysis of Staphylococcus aureus, producing a spade-shaped pattern of complete haemolysis which extends across the streak of S. aureus.
CAMP test-positive
Generally acquired by inhalation of dust contaminated with R. equi.
Suppurative bronchopneumonia of foals
Many species with branching filaments; anaerobic or facultatively anaerobic; morphologically heterogenous; nonmotile; MZN negative or positive.
-relatively slow growth on laboratory media
- opportunistic pathogens
Genus Actinomyces
Invasion of the mandible and, less commonly, the maxilla of cattle by Actinomyces bovis causes a chronic rarefying osteomyelitis.
Bovine actinomycosis (lumpy jaw)
Pinhead-sized yellowish ‘sulphur granules’ are found
Bovine actinomycosis (lumpy jaw)
Aerobic, nonmotile saprophytic actinomycetes
-causes opportunistic infection, associated with immunosuppression or may follow after a heavy challenge.
Genus Nocardia
Genus Dermatophilus Species
D. congolensis
D. dermatonomus
D. pedis
streptotrichosis in cattle
D. congolensis
mycotic dermatitis in sheep
D. dermatonomus
strawberry foot rot in sheep
D. pedis
characterized by formation of crush and tendency to spread over large areas
Rain scald
Form of disease in sheep
dermatitis of wool-covered areas, face (lumpy wool)
dermatitis of hairy parts of the face and strotum
dermatitis of lower leg (foot rot)