Bacterial Genetics

Question 1

The rate of DNA replication of E. coli is determined by rate of ______, not growth rate

Answer 1

Initiation

Question 2

Nucleotide is changed

Answer 2

Base substitution

Question 3

Nucleotide change results in stop codon

Answer 3

Nonsense

Question 4

Nucleotide change results in different amino acid

Answer 4

Missense mutation

Question 5

Transfer of plasmid DNA through sex pili

Answer 5

Conjugation

Question 6

Bacteria phage (virus) accidentally inject bacterial DNA into host bacterial cell

Answer 6

Transduction

Question 7

DNA that can insert or excise itself from the chromosome or plasmid

Answer 7

Transposable elements

Question 8

Ability to take up DNA from environment, mediated by quorum sensing

Answer 8

Natural Transformation

Question 9

Bacteria sense their environment through _______, allowing them to control which proteins are made and how much

Answer 9

Quorum Sensing

Question 10

TCS (Two Component Regulatory System)- regulates genes in response to an ______ signal

Answer 10

Environmental

Question 11

RNA polymerase binds to ____ located upstream of gene start codon

Answer 11

Promoter

Question 12

Random mutations are introduced creating a mutant library that can be screened

Answer 12

Random Mutagenesis

Question 13

A gene is replaced by an antibiotic marker

Answer 13

Allelic Replacement

Question 14

A gene from a pathogenic organism is expressed in a non-pathogenic strain to see if the strain becomes virulent (I.e. Host cell invasion)

Answer 14

Complementation

Question 15

Ability to manipulate genes- make mutations or knockout genes

Answer 15

Functional Genomics

Question 16

Uses a gene chip (has a fragment of each gene of a bacteria), hybridizes two populations of differently labeled RNA samples to determine the differences in level of expression

Answer 16

Microarray

Question 17

Total RNA is isolated and sequenced using Illuminia technology. RNA reads are counted to determine differential gene expression. (More modern than Microarray)

Answer 17

RNA sequencing