bacterial cultures Flashcards

1
Q

what is chocolate agar used for ?

A

h influenza

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2
Q

what are the growth requirements for H.influenza ?

A

NAD factor 5
Hemin factor 10

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3
Q

what is the only condition in which hib can ow on blood agar ?

A

if staph aureus also grows on the same dish of blood agar

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4
Q

what is used for thee differentiation between different strep species ?

A

blood agar
through the different modes ooff hemolysis

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5
Q

what are the different types of hemolysis seen on blood agar and what are the species associated with each type ?

A

beta hemolysis - S.pyogens , S. agalactia
alpha hemolysis - S.pneumonia, S.viridans
gamma hemolysis - enterroccoccus , strep bovis

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6
Q

most strep aree gram what

A

gram negative

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7
Q

what type of hemolysis is associated with staph aureus ?

A

beta hemolytic

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8
Q

what type of bacteria is seen using thayer martin media ?

A

neisseria

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9
Q

what type of media is used too see bordatella pertussis ?

A

bordet-gengou agar
also called potato agar

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10
Q

what media is used for the identification of diphtheria
and which is used too isolate and differentiate diphtheria ?

A

loeffler’s media for identification.
tellurite for differentiation

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11
Q

what medium is used to grow mycobacterium ?

A

lowenstein jensen agar
middlebrook mdium

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12
Q

what medium is used to grow mycoplasma ?

A

eaton agar

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13
Q

what medium is used for gram negative bacteria ?

A

mackonkeyy agar

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14
Q

how do we differentiate between lactose and non lactose fermenting gram negative bacteria ?

A

mackonkey agar

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15
Q

what medium is very close to mackonkey agar ?

A

eosin methylene blue agar

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16
Q

what is sorbitol mackonkey agar used for ?

A

the detection of E Coli 0157:H7

17
Q

what medium is used exclusively for shigella ?

A

buttered charcoal yeast extracct

18
Q

what is sabourads agar used for ?

A

the detection of fungi

19
Q

what bugs are grown in charcoal yeast extract ?

A

legionella
pasteurella
brucella
francisella
ay 7aga ella

20
Q

how is Thayer martin made to exclusivly grow neisseria ?

A

by addition of vancomycin
trimethoprim
colistin
nystatin

21
Q

what dos mycoplasma need to grow ?

A

grows in eaton agar and requires cholesterol

22
Q

what is the charcoal yeast extract buffered with ?

A

iron and cysteine