Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Microbiology and immunology > Bacterial culture (midterm) > Flashcards

Bacterial culture (midterm) Flashcards

1
Q

What are examples of bacteria that cannot be cultured in vitro?

A

Chlamydia
Rickettsia
Mycobacterium leprae
Treponema pallidum

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What conditions do Chlamydia and Rickettsia need?

A

Tissue culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What conditions do Mycobacterium leprae and Treponema pallidum need?

A

Animal infection

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What are the types of simple media?

A

Nutrient broth
Nutrient agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What are the components of nutrient broth?

A

Peptone
Meat extract
NaCl

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What are the components of nutrient agar?

A

Nutrient broth (peptone, meat extract, NaCl)
2% agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What are the types of special media?

A

Enriched media
Differential media
Selective media
Transport media
Anaerobic media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What are the types of enriched media?

A

Blood agar
Chocolate agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What characteristic of bacteria necessitates enriched media?

A

Fastidious/exacting bacteria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What are the components of blood agar?

A

Nutrient agar
Mammalian blood (usually horse or sheep) at 5–10%

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What are the components of chocolate agar?

A

Blood agar slowly heated to 80ºC causing hemolysis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What is an example of a bacterium requiring chocolate agar?

A

Haemophilus influenzae

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What are the typical constituents of selective media?

A

Antibiotics
Dyes
Chemicals
Specific pH

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What are the components of Thayer–Martin medium?

A

Vancomycin: kills G+ bacteria
Colistin: kills most G– bacteria
Nystatin: kills most fungi
Trimethoprim: inhibits most G– negative bacteria, especially swarming Proteus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What bacteria is selected for by Thayer–Martin medium?

A

Neisseria gonorrhoeae

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What is the function of vancomycin in Thayer–Martin medium?

A

Kills G+ bacteria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

What is the function of colistin in Thayer–Martin medium?

A

Kills most G– bacteria (except N. gonorrhoeae)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

What is the function of nystatin in Thayer–Martin medium?

A

Kills most fungi

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

What is the function of trimethoprim in Thayer–Martin medium?

A

Inhibits G– bacteria, especially swarming Proteus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

What medium is selective for Neisseria gonorrhoeae?

A

Thayer–Martin medium

21
Q

What type of bacteria is eosin methylene blue medium selective for?

A

G– bacteria

22
Q

How does eosin methylene blue medium function?

A

Methylene blue inhibits the growth of G+ bacteria

23
Q

What medium is selective for G– bacteria?

A

Eosin methylene blue

24
Q

What are the components of Campylobacter agar?

A

Bacteriological charcoal
Cefoperazone
Amphotericin B

25
Q

What is Campylobacter agar used for?

A

Isolation of Campylobacter jejuni from fecal or rectal swabs

26
Q

What medium is selective for Campylobacter jejuni?

A

Campylobacter agar

27
Q

What are the components of Lowenstein–Jenson medium?

A

Penicillin
Nalidixic acid
Malachite green

28
Q

How does Lowenstein–Jenson medium function?

A

Inhibits the growth of G+ and G– bacteria, except Mycobacterium tuberculosis

29
Q

What bacterium does Lowenstein–Jenson medium select for?

A

Mycobacterium tuberculosis

30
Q

What medium is selective for Mycobacterium tuberculosis?

A

Lowenstein–Jensen medium

31
Q

What are the types of differential media?

A

MacConkey agar
CLED agar
TCBS agar
XLD agar

32
Q

How does MacConkey medium function?

A

Lactose fermenters: colorless → pink colonies
Non-lactose fermenters: colorless colonies

33
Q

What feature does MacConkey agar differentiate?

A

Lactose fermentation

34
Q

What medium differentiates lactose fermenters and non-lactose fermenters?

A

MacConkey agar

35
Q

Which bacteria appear pink in MacConkey’s agar

A

E. coli
Klebsiella

36
Q

Which bacteria remain pale in MacConkey’s agar?

A

Salmonella
Shigella

37
Q

What is CLED agar used for?

A

Cultivation of pathogens from urine specimens, inhibiting swarming Proteus

38
Q

What medium is used for urine specimens?

39
Q

What is TCBS agar used for?

A

Isolation of Vibrio cholerae and V. parahaemolyticus

40
Q

What medium is used to isolate Vibrio cholerae and V. parahaemolyticus?

41
Q

What is XLD agar used for?

A

Recovery of Salmonella and Shigella species

42
Q

What medium is used to cultivate recovering Salmonella and Shigella species?

43
Q

What are examples of transport media?

A

Stuart’s medium
Buffered glycerol saline

44
Q

What type of medium is Stuart’s medium?

A

Transport medium

45
Q

What type of medium is buffered glycerol saline?

A

Transport medium

46
Q

What are examples of anaerobic media?

A

Robertson’s cooked meat medium
Thioglycolate broth medium

47
Q

What type of medium is Robertson’s cooked meat medium?

48
Q

What type of medium is thioglycolate broth medium?

Microbiology and immunology (33 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions