Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

Microbiology and immunology > Bacterial culture (midterm) > Flashcards

Bacterial culture (midterm) Flashcards

1
Q

What are examples of bacteria that cannot be cultured in vitro?

A

Chlamydia
Rickettsia
Mycobacterium leprae
Treponema pallidum

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What conditions do Chlamydia and Rickettsia need?

A

Tissue culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What conditions do Mycobacterium leprae and Treponema pallidum need?

A

Animal infection

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What are the types of simple media?

A

Nutrient broth
Nutrient agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What are the components of nutrient broth?

A

Peptone
Meat extract
NaCl

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What are the components of nutrient agar?

A

Nutrient broth (peptone, meat extract, NaCl)
2% agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What are the types of special media?

A

Enriched media
Differential media
Selective media
Transport media
Anaerobic media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What are the types of enriched media?

A

Blood agar
Chocolate agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What characteristic of bacteria necessitates enriched media?

A

Fastidious/exacting bacteria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What are the components of blood agar?

A

Nutrient agar
Mammalian blood (usually horse or sheep) at 5–10%

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What are the components of chocolate agar?

A

Blood agar slowly heated to 80ºC causing hemolysis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What is an example of a bacterium requiring chocolate agar?

A

Haemophilus influenzae

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What are the typical constituents of selective media?

A

Antibiotics
Dyes
Chemicals
Specific pH

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What are the components of Thayer–Martin medium?

A

Vancomycin: kills G+ bacteria
Colistin: kills most G– bacteria
Nystatin: kills most fungi
Trimethoprim: inhibits most G– negative bacteria, especially swarming Proteus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What bacteria is selected for by Thayer–Martin medium?

A

Neisseria gonorrhoeae

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What is the function of vancomycin in Thayer–Martin medium?

A

Kills G+ bacteria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

What is the function of colistin in Thayer–Martin medium?

A

Kills most G– bacteria (except N. gonorrhoeae)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

What is the function of nystatin in Thayer–Martin medium?

A

Kills most fungi

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

What is the function of trimethoprim in Thayer–Martin medium?

A

Inhibits G– bacteria, especially swarming Proteus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

What medium is selective for Neisseria gonorrhoeae?

A

Thayer–Martin medium

21
Q

What type of bacteria is eosin methylene blue medium selective for?

A

G– bacteria

22
Q

How does eosin methylene blue medium function?

A

Methylene blue inhibits the growth of G+ bacteria

23
Q

What medium is selective for G– bacteria?

A

Eosin methylene blue

24
Q

What are the components of Campylobacter agar?

A

Bacteriological charcoal
Cefoperazone
Amphotericin B

25
What is Campylobacter agar used for?
Isolation of *Campylobacter jejuni* from fecal or rectal swabs
26
What medium is selective for *Campylobacter jejuni*?
Campylobacter agar
27
What are the components of Lowenstein–Jenson medium?
Penicillin Nalidixic acid Malachite green
28
How does Lowenstein–Jenson medium function?
Inhibits the growth of G+ and G– bacteria, except *Mycobacterium tuberculosis*
29
What bacterium does Lowenstein–Jenson medium select for?
*Mycobacterium tuberculosis*
30
What medium is selective for *Mycobacterium tuberculosis*?
Lowenstein–Jensen medium
31
What are the types of differential media?
MacConkey agar CLED agar TCBS agar XLD agar
32
How does MacConkey medium function?
Lactose fermenters: colorless → pink colonies Non-lactose fermenters: colorless colonies
33
What feature does MacConkey agar differentiate?
Lactose fermentation
34
What medium differentiates lactose fermenters and non-lactose fermenters?
MacConkey agar
35
Which bacteria appear pink in MacConkey's agar
*E. coli* *Klebsiella*
36
Which bacteria remain pale in MacConkey's agar?
*Salmonella* *Shigella*
37
What is CLED agar used for?
Cultivation of pathogens from urine specimens, inhibiting swarming *Proteus*
38
What medium is used for urine specimens?
CLED agar
39
What is TCBS agar used for?
Isolation of *Vibrio cholerae* and *V. parahaemolyticus*
40
What medium is used to isolate *Vibrio cholerae* and *V. parahaemolyticus*?
TCBS agar
41
What is XLD agar used for?
Recovery of *Salmonella* and *Shigella* species
42
What medium is used to cultivate recovering *Salmonella* and *Shigella* species?
XLD agar
43
What are examples of transport media?
Stuart's medium Buffered glycerol saline
44
What type of medium is Stuart's medium?
Transport medium
45
What type of medium is buffered glycerol saline?
Transport medium
46
What are examples of anaerobic media?
Robertson's cooked meat medium Thioglycolate broth medium
47
What type of medium is Robertson's cooked meat medium?
Anaerobic
48
What type of medium is thioglycolate broth medium?
Anaerobic

Microbiology and immunology (33 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions