Bacillus Flashcards
shape and arrangement
rods
pairs or long chains
endospore survival
more than 50 years
gram, size, spore, respiration, growth, motility, catalase, oxidase, pathogenic
-gram positive
-large
-endospores produced
-aerobes or facultative anaerobe
-growth on non enriched media
-most motile
-most catalase positive
-most oxidase negative
-majority non pathogenic environmental organisms
B anthracis motility, hemolysis, lactithinase, gamma phage
-non motile
-non hemolytic on sheep blood agar
-weak and slow on lecthinase activity egg yolk agar
-effect of gamma phage – lysis
colonie appearance of B anthracis
-5mm diameter
-flat
-dry
-greyish
B cereus motility, hemolysis, lecithinase, pathogenicity, gamma phage, colonies
-motile
-hemolytic on sheep blood agar
-strong and rapid lecithinase activity on egg yolk agar
- pathogenicity – no effect
effect of gamma phage – lysis rare
-greenish tinge to colonies
B licheniform colonies
dull, wrinkled, strongly adherent to agar
clinical manifestation of B anthracis
-fatal peracute or acute septicemic anthrax
-subacute anthrax with edematous swelling in pharyngeal region
-septicemia with colic and enteritis
-skin, pulmonary, intestinal forms
clinical manifestation of B cereus
-mastitis (rare)
-food poisoning, eye infections
clinical manifestations of B licheniformis
-sporadic abortions in cow and sheep
pathogenesis
-capsule provides resistance to phagocytosis
-protective antigens , oedema factor, lethal factor
-oedema factor targets neutrophils
-lethal factor targets macrophages, dendritic cells, neutrophils
which species more susceptible
sheep and cattle
clincial signs
-fever
-depression
-congested mucosae and pectechiae
-abortions
what to do when animals die
-do NOT do necropsy
-report to government they will take care of it
lesions
-rapid bloating
-incomplete rigor mortis
-ecchymotic hemorrhage and oedema
-dark unclotted blood and blood stained fluids in cavities
-large and soft spleen
