Actinomyces, Arcanobacterium (Trueperella), Actinobaculum

Question 1

actinomyces species – respiration, filament production, ZN stain, sabouraud dextrose agar, habitat, site of lesions

Answer 1

-anaerobic or facultatively anaerobic
-capnophilic
-no aerial filament production
-no modified ZN staining
-no growth on sabouraud dextrose agar
-usual habitat – nasopharyngeal and oral mucosa
-site of lesions – many tissues including bone

Question 2

Trueperella pyogenes – respiration, filament production, ZN stain, sabouraud dextrose agar, habitat, site of lesions

Answer 2

-facultative anaerobic
-capnophilic
-no aerial filament production
-no ZN staining
-no growth on sabouraud dextrose agar
-usual habitat – nasopharyngeal mucosa of cattle, sheep, pigs
-site of lesions – soft tissues

Question 3

actinobaculum suis – respiration, filament production, ZN staining, sabouraud dextrose agar, habitat, site of lesions

Answer 3

-anaerobic
-no aerial filament production
no modified ZN staining
-no growth on sabouraud dextrose agar
-usual habitat – prepuce and preputial diverticulum of boars
-site of lesions – urinary tract of sows

Question 4

nocardia species – respiration, filament production, ZN staining, sabouraud dextrose agar, habitat, site of lesions

Answer 4

-aerobic
-yes aerial filament production
-yes modified ZN staining
-yes growth on sabouraud dextrose agar
-usual habitat – soil
-site of lesion – thoracic cavity, skin, other tissues

Question 5

dermatophilus congolensis – respiration, filament production, ZN staining, sabouraud dextrose agar, habitat, site of lesions

Answer 5

-aerobic
-capnophilic
-no aerial filament production
-no modified ZN staining
-no growth on sabouraud dextrose agar
-usual habitat – skin of carrier animals, scabs from lesions
-site of lesions – skin

Question 6

trueperella pyogenes diseases

Answer 6

-cattle, sheep pigs – abscessation, mastitis, suppurative pneumonia, endometritis, pyometra, arthritis, umbilical infections

Question 7

actinomyces hordeovulneris diseases

Answer 7

-dogs – cutaneous and visceral abscessation, pleuritis, peritonitis, arthritis

Question 8

actinomyces bovis diseases

Answer 8

-cattle – bovine actinomycosis (lumpy jaw)

Question 9

actinomyces viscous diseases

Answer 9

-dogs – canine actinomyces, cutaneous pyogranulomas, pyothorax, proliferative pyogranulomatous pleural lesions, disseminated lesions
-horses – cutaneous pustules
-cattle – abortions

Question 10

actinomyces species (unclassified) disease

Answer 10

-pigs – pyogranulomatous mastitis
- horses – poll evil, fistulous withres

Question 11

actinomyces suis diseases

Answer 11

-pigs – cystitis, pyelonephritis

Question 12

phenotype – actinomyces bovis

Answer 12

-filamentous branching, some short forms
-anaerobic + CO2
-+/- hemolysis on sheep blood agar
-no catalase production
-no pitting on loefflers serum slope
-sulfur granules in pus

Question 13

phenotype – actinomyces viscosus

Answer 13

-filamentous branching, short forms
-10% CO2
-no hemolysis on sheep blood agar
-yes catalase production
-no pitting on loefflers serum slope
white granules in pus

Question 14

phenotype – actinomyces hordeovulneris

Answer 14

-filamentous branching, short forms
-10% CO2
-+/- hemolysis on sheep blood agar
-yes catalase production
-no pitting on loefflers serum slope
-no granules in pus

Question 15

phenotype – trueperella pyogenes

Answer 15

-coryneform
-aerobic
-yes hemolysis on sheep blood agar
-no catalase production
-yes pitting on loefflers serum slope
-no granules in pus

Question 16

phenotype – actinobaculum suis

Answer 16

-coryneform
-anaerobic
-+/- hemolysis on sheep blood agar
-no catalase production
-no pitting on loefflers serum slope
-no granules in pus