B1.1.4 - Electron microscopy Flashcards
What is different about electron microscope than light microscopes?
Light microscopes use light to produce and image but electron microscopes use electrons.
What are the 2 types of electron microscopes?
Transmission electron microscopes
Scanning electron microscopes
What are the advantages of electron microscopes?
It is has a high resolution and the any colour images can be produced.
What are the disadvantages of electron microscopes?
It is expensive to buy and operate
It is large and difficult to move
Sample preparation is complex
The specimens have to be dead
How does a TEM work?
A beam of electrons pass through a very thin slice of the sample. The beam is focused to produce an image
How does an SEM work?
They send a beam of electrons across the surface of a specimen. The reflected electrons are collected to produce an image
What kind of images do TEMs produce?
The produce the most magnified images
What kinds of images does an SEM produce?
It produces a three dimensional image of a surface
What is the resolution of a electron microscope?
Up to 0.1 nm