B1.1.4 - Electron microscopy Flashcards

1
Q

What is different about electron microscope than light microscopes?

A

Light microscopes use light to produce and image but electron microscopes use electrons.

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2
Q

What are the 2 types of electron microscopes?

A

Transmission electron microscopes

Scanning electron microscopes

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3
Q

What are the advantages of electron microscopes?

A

It is has a high resolution and the any colour images can be produced.

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4
Q

What are the disadvantages of electron microscopes?

A

It is expensive to buy and operate
It is large and difficult to move
Sample preparation is complex
The specimens have to be dead

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5
Q

How does a TEM work?

A

A beam of electrons pass through a very thin slice of the sample. The beam is focused to produce an image

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6
Q

How does an SEM work?

A

They send a beam of electrons across the surface of a specimen. The reflected electrons are collected to produce an image

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7
Q

What kind of images do TEMs produce?

A

The produce the most magnified images

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8
Q

What kinds of images does an SEM produce?

A

It produces a three dimensional image of a surface

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9
Q

What is the resolution of a electron microscope?

A

Up to 0.1 nm

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