Assay for Enzymatic Activity Flashcards
(Reference method) in lipase assay method
A. Tanzer
B.chery
B. Chery
Endpoint of cherry and tietz
A. Phenolphthalein
B.thymolphtalein+ veronal
C.triolein
D. Olive oil
D. Fatty acid (oleic)
Fatty acid oleic
Indicator of cherry
A. Phenolphthalein
B.thymolphthalein + veronal
Phenolphthalein
Indicator of tietz
A. Phenolphthalein
B.thymolphthalein + veronal
B. Thymolphthalein + veronal
Substrate of tietz and cherry crandall
50% olive oil (triolein- a more pure form of triglyceride)
Cherry and tietz titrating agent
0.4N NaOH
Cherry Crandall end color
Pink
Tietz end color
Blue
Principle: the amount of the light block by the particles insoluble substances or agents in the sample
Turbidemetry method
The reagent use in turbidimetry method
Triglycerides
Turbidimetry Method
——- in absorbance is measured at 340nm
A.decrease
B.imcrease
Decrease
The activity if lipase to triacylglycerol is—- proportional to the absorbance reading of the solution
A. Inversely
B. Directly
A
