Aspartate Aminotransferase (AST)

Question 1

Ast is also known as?

A. Serumgluta- oxaloacetictransaminase
B. Serum glutamic-oxaloacetictransaminase
C. Serum glutamic-pyruvic transaminase

Answer 1

B. Serum glutamic-oxaloacetictransaminase

Question 2

AST

Catalyzes the transfer of an amino group between ____ and _____

A. Alanine and 2 monomer
B.alanine and pyruvic acids
C. Alanine and aspartate
D. aspartate and α-keto acids

Answer 2

D. aspartate and α-keto acids

Question 3

AST
Involved in synthesis and degradation ———- ) of AA in the liver

A. anabolism & catabolism
B. y-glutamyl peptides
C. phosphomonoester bonds
D. p-nitrophenol

Answer 3

A. anabolism & catabolism

Question 4

AST

For aminotransferases, the coenzyme required is _______
A. VITAMIN B12
B. VITAMIN B6
C. pyridoxal phosphate
d. hydrogen transfer enzyme

Answer 4

B AND C

Question 5

TRUE OR FALSE AST

Enzymes does similar catalytic activity but differ in physical,
biochemical and immunologic properties

Answer 5

TRUE

Question 6

most abundant and predominant AST isoenzyme in the healthy human serum

A. Cytoplasmic AST
B. Mitochondrial AST

Answer 6

A. Cytoplasmic AST

Question 7

INCREASE In serum indicates possible cell necrosis/ severe damage to the
cell

A. Creatine Kinase (CK)
B. Mitochondrial AST
C. Lactate Dehydrogenase

Answer 7

B. Mitochondrial AST

Question 8

After Acute Myocardial Infarction, AST levels begin to rise in____hours, peak at 24 hours and return to normal in 5 days

A. 5 TO 6 HRS
B. 6-8 HOURS
C.8 HOURS
D. 6-9 HOURS

Answer 8

B. 6-8 HOURS

Question 9

Also ↑ in hepatocellular and skeletal muscle disease

A. AST
B.GGT
C.ALT
D.CK

Answer 9

A. AST

Question 10

Released to greater degree in cases of chronic disorders of the liver with
progressive damage

A. AST/SGOT
B.AST/GGT
C.ALT
D.CK

Answer 10

A. AST/ SGOT

Question 11

type of cofactor that serves as second substrate for enzymatic reaction

Answer 11

COENZYMES

Question 12

Tightly bound enzyme to coenzyme

Answer 12

Prosthetic group

Question 13

example of COENZYMES

Answer 13

NAD
NADP

Question 14

NAD MEANS?

Answer 14

Nicotinamide adenine dinucleotide

Question 15

IF the NAD is used as coenzymes, it will convert into 2 forms when acted
by oxidoreductase enzyme by what kind of reaction?

Answer 15

REDOX REACTION

Question 16

REDUCED FORM

Answer 16

NADH

Question 17

OXIDIZED FORM

Answer 17

NAD without H

Question 18

WHAT METHOD IS USED FOR AST?

A. Forward Reaction
B(Oliver-Rosalki)
C. Wacker Method
D. Karmen Method

Answer 18

D. Karmen Method

Question 19

Uses malate dehydrogenase (MD) as the coupling/secondary/indicator enzyme

A.karmen method, SGOT
B. AST, Szaz Assay
C. Ast, karmen method
D. CK, Oliver-Rosalk

Answer 19

A or c

Question 20

malate dehydrogenase (MD) as the coupling/secondary/indicator enzyme and monitors decrease in absorbance at —— nm?

A. 360
B.340
C. 405-420

Answer 20

B

Question 21

KARMEN METHOD

Falsely increased enzyme activity in—— sample.

A. Icteric sample
B. Hemolyzed
C.lysed sample
D. Clotted sample

Answer 21

B. Hemolyzed

Question 22

Reference value of karmen method in AST?

A. 30-90 U/L
B. 5-30 U/L
C.5-90 U/L
D. 50-260U/L

Answer 22

A. 30-90 U/L

Question 23

Chemical reaction PRODUCT of AST

A. Malate + NAD
B. Malate + NADPH

Answer 23

A. Malate + NAD

Question 24

coupling/secondary/indicator enzyme in oxidoreductase class

Answer 24

malate dehydrogenase (MD)