Alanine Aminotransferase (ALT) Flashcards
Alanine Aminotransferase (ALT) also known as?
A. Serum glutamic-pyruvic transaminase (SGPT)
B. Alkaline Orthophosphoric
C. Ast
D. SGOT
A. Serum glutamic-pyruvic transaminase (SGPT)
Alanine Aminotransferase (ALT)
Catalyzes the transfer of an amino group between —- and —-
A. Alanine and α-keto acids
B. aspartate and α-keto acids
C. Alanine and α-ketoglutarate
C. Alanine and α-ketoglutarate
Increased in hepatocellular disorders (considered as liver specific enzyme)
A. Ast
B. Ck-MB
C. ALT
D. SGOT
ALT
Enumerate the 4 uses of ALT
- acute inflammatory conditions of the liver
- hepatitis treatment and effects of drug therapy specially among patients with chronic diseases
- screening test for post transfusion hepatitis and occupational toxic exposure
- screen blood donors if donor is suspected of having hepatitis B.
”AI HDC PT OTE SB
Shows the result of AST/ALT ratio result
De Ritis Ratio
Differentiates the cause of hepatic disorder
De retis ratio
Ratio >1
A. viral in origin; ALT is greater
B. non-viral origin; AST is greater
B. non-viral origin; AST is greater
viral in origin; ALT is greater
A. Ratio <1
B. Ratio >1
A. Ratio <1
The coupling enzyme in Alanine Aminotransferase (ALT)
A. alcohol dehydrogenase
B. Lactate Dehydrogenase
C. malate dehydrogenase (MD)
B. Lactate Dehydrogenase
The enzymatic absorbance in ALT IS. — in absorbance at 340nm.
A. Decrease
B.increase
Decrease
ALT reference range
A. 6-37 U/L
B. 5-30 U/L
C.6-40 U/L
A. 6-37 U/L
Reaction product in ALT
A.LACTATE + NAD+
B.LACTATE + NADH+
A.LACTATE + NAD+
Major organ affected in AST/ SGOT
A. Heart
B.lungs
C.kidney
Heart
Major organ affected in ALT/SGPT
A. Heart
B. Skeletal
C. Muscle
D.liver
Liver
Substrate in ALT
A. Alanine Ketoglutaric Acid
B Aspartic Alpha Ketoglutaric Acid
a. Alanine Ketoglutaric Acid
End Products of ALT
A. Glutamic acid + Pyruvic acid
B. Glutamic acid + Oxaloacetate
A. Glutamic acid + Pyruvic acid
End Products of AST
A. Glutamic acid + Pyruvic acid
B. Glutamic acid + Oxaloacetate
B. Glutamic acid + Oxaloacetate
Color Developer in AST/ALT
A. y-glutamyl
B. 2,4 DNPH (Dinitrophenylhydrazine)
C.2, 4 DNPH ( Dinitrophenylbenzide)
D. p-Nitroaniline
B. 2,4 DNPH (Dinitrophenylhydrazine)
Color Intensifier in AST/ alt
0.4 NaOH
Methods used in ast/ alt
A. Reitman and Frank (colorimetric method)
B. Wrobleuski La Due (colorimetric method)
C. Reitman and Frankel (colorimetric method)
D. B. Wrobleuski Due (colorimetric method)
C. Reitman and Frankel (colorimetric method)
