App 12 - Blood Cells (Pre-Lab)

Question 1

what stain is used for blood cells?

Answer 1

wright stain - eosin for acid, and methylene blue for base

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erythrocyte

Centrally, red cells possess an area of pallor (which reflects their actual biconcave nature) that accounts for approximately one-third of the diameter of the cell. The area of central pallor correlates with the hemoglobin content of the cell and red cells with a normal-sized area of central pallor are called normochromic. Erythrocytes with an enlarged area of central pallor are hypochromic, while those that lack an area of central pallor are hyperchromic.

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Question 13

what stain is used for bone marrow?

Answer 13

wright stain again, eosin acid methylene blue base

Question 14

What do bone marrow cells look like as they differentiate?

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erythroblast (pronormoblast). Erythroblasts are
large cells, with large centrally-placed, round nuclei with dispersed nuclear chromatin (often with one or
more nucleoli visible) and a scant amount of deeply basophilic cytoplasm.

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Following the loss of its nucleus, the red cell precursor is released into the blood as a reticulocyte and then, once hemoglobin synthesis is completed, it becomes a mature erythrocyte.

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myeloblast, which in normally accounts for less than
1% of the total marrow cellularity. The morphologic features of myeloblasts have been described
above. Morphologically, when compared to erythroblasts, the nuclei of myeloblasts are not as perfectly
round and often touch the edge of the cell (rather than being centered) and their cytoplasm is not quite
as basophilic (but these are subtle features).

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myelocyte. It is at the myelocyte stage that cells begin to
develop specific differentiation. In cells destined to become neutrophils, the primary granules of the
promyelocyte are replaced by pink-staining granules typical of neutrophils.

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Question 34

How is a bone marrow biopsy stained?

Answer 34

H&E