AO1 - Unit 5 Flashcards
Which part of the leaf cell absorbs light energy?
in chloroplast
Where does the light dependent stage occur?
Grana/ Thylakoid membranes
What is a granum?
Stack of Thylakoids
Name the 2 products of the LDR that are used in the LIR?
ATP and NADPH
What does photolysis of water produce?
electrons, protons, and oxygen
What happens during photoionisation?
electrons in chlorophyll are excited and lost
Name the enzyme that catalyses the reaction to synthesis ATP
ATP synthase
What is formed in the LIR?
Glucose (plus glycerol, fatty acids, amino acids)
Give another name for the light independent step?
Calvin cycle
What provides the energy for the LIR?
ATP
Where does the light independent reaction occur?
Stroma of the chloroplast
What is the first step of the LIR?
Fixation of carbon dioxide to Ribulose bisphosphate by the enzyme RUBISCO
How many carbon atoms are in Ribulose bisphosphate?
5
How is glycerate 3 phosphate (GP) made?
splitting of an unstable 6C intermediate
What is the role of rubisco?
Fixing carbon dioxide to RuBP
How is TP made?
reduction of Glycerate phosphate using ATP and NADPH
What is the role of red NADP from the LD reaction?
To provide the H to reduce Glycerate phosphate to Triose phosphate
What is the role of ATP?
To provide the energy to reduce Glycerate phosphate to triose phosphate
What 2 substances can be made from TP
Glucose and ribulose bisphosphate (recycled)
What is a limiting factor?
The factor, which if it increases, will increase the rate of the reaction ? it’s in shortest supply.
What environmental factors limit the rate of photosynthesis?
Light intensity, carbon dioxide concentration, temperature (enzymes ? mainly in LIR)
What is chromatography?
Separation of a mixture according to their solubility in a mobile phase
What is a dehydrogenase?
Enzyme catalysing the transfer of hydrogen in redox reactions
What is oxidation?
loss of electrons, hydrogen or gain of oxygen
What is reduction?
gain of electrons, hydrogen or loss of oxygen
What is a coenzyme?
A molecule that aids the function of an enzyme ? often by transferring groups ? hydrogen or acetyl groups
What does respiration produce?
aerobic - carbon dioxide, water, ATP anaerobic (plants & fungi) ethanol, carbon dioxide, ATP anaerobic (animals & bacteria) lactic acid + ATP
What is the first stage of aerobic and anaerobic respiration?
Glycolysis
Where does glycolysis occur?
Cytoplasm
What is the first step in glycolysis?
Phosphorylation of glucose
What are the products of glycolysis?
2 pyruvate, 2ATP and 2NADH
What is formed initially from the phosphorylated glucose?
Fructose 1,6 diphosphate which then splits into triose phosphate
How many carbon atoms in pyruvate?
3
What type of reaction converts TP into pyruvate?
oxidation
What happens to pyruvate in anaerobic respiration?
reduced to lactate (animals & bacteria) Decarboxylated to ethanal which is then reduced to ethanol (plants and fungi)
In aerobic conditions what happens to the pyruvate?
actively transported into the mitochondrion
Where does the link reaction happen?
Mitochondrial matrix