AO1 - Unit 2 Flashcards
All cells arise from other ______________
cells
Prokaryotic cells form new cells by ______________ _______________
binary fission
Name two types of cell division.
mitosis and meiosis
What is the role of the cell surface membrane?
Selectively permeable to control the exchange of material
Give two types of molecule found in plasma membranes.
phospholipids, proteins
Which type of cell has internal membranes?
eukaryotic cell
What is the role of the nucleus?
stores DNA in the form of chromatin
What is the role of the nucleolus?
makes ribosomes
What is the role of chloroplasts?
absorb light energy for photosynthesis
Where are chloroplasts found?
plants and algae
What is role of Golgi apparatus?
modifies and packages proteins, adds carbohydrates to form glycoproteins
What type of cell will have a lot of Golgi apparatus?
cells which secrete proteins or glycoproteins such as beta cells of the islets of langerhans
What is the role of lysosomes?
contain lysosymes which hydrolyse pathogens or worn out organelles
What is the difference between RER and SER?
rough contains ribosomes on surface, smooth does not
What is the role of the RER?
transports proteins made at ribosomes
What is the role of the SER?
synthesise, store and secrete lipids
What type of cell have a cell wall?
prokaryotes and some eukaryotes - plants, fungi
Eukaryotic cells undergo what to become suited to a particular job?
differentiation/specialisation
Name a cell that has microvilli.
epithelial cells of the ileum
Where are cristae found?
mitochondria
What is the role of the cell vacuole?
storage of water/sugars needed for growth, waste disposal and turgidity
Give 4 differences between prokaryotic and eukaryotic cells.
linear DNA vs circular DNA / nucleus vs no nucleus / membrane bound organelles vs none (like mitochondria, chloroplast, golgi body) / 80S ribosomes vs 70S ribosomes
What type of cell has a plasmid?
prokaryotes / bacteria
What type of particle is described as acellular and non-living?
virus
What stain is used to locate starch grains in a plant cell?
iodine
How is magnification calculated?
magnification = image size / actual size
How many micrometres in a mm?
1000
What is resolution?
minimum distance two objects can be seen to be distinct from one another
Explain why electron microscope has a greater resolving power than optical microscope.
shorter wavelength of electrons
In microscopy, what is an artefact?
something introduced to the image during preperation, excess stain for example, which is not naturally there
What is cell fractionation used for?
separate out organelles for study
What is homogenisation?
blending or breaking open cells to release contents
Give 3 properties of the solution used to homogenise the tissue
isotonic, ice cold and pH buffered
Why is the mixture filtered after homogenisation?
remove any debris which may have similar mass to organelles
Why do organelles separate out during centrifugation?
based on mass - heavier will go to bottom at lower speeds
After centrifugation what is left at the bottom of the tube?
pellet - heaviest organelles first
What do we call the fluid above it?
supernatant
In what order will organelles separate out?
nucleus, mitochondria/chloroplast, lysosomes, endoplasmic reticulum, ribosomes
During which stage of the cell cycle does DNA replication occur?
interphase - S phase
Which part of the cell cycle produces 2 identical daughter cells?
mitosis
How would you describe DNA replication?
semi-conservative
Where do centromeres attach during mitosis?
spindle
Name the stages of mitosis
prophase, metaphase, anaphase, telophase