Antibody Stucture And Function- Lecture 9/29/21

Question 1

Complementary Determining Region (CDR)

Answer 1

Also called the hypervariable region, 3 spots in each variable region that recognize specific isotope

Question 2

Five main Ig Isotypes

Answer 2

IgM (mu)
IgD (delta)
IgG (gamma)
IgA (Alpha)
IgE (epsilon)

Question 3

Ig’s with subclasses

Answer 3

IgG (4) and IgA (2)

Question 4

Light chain isotopes

Answer 4

Kappa and Lambda, don’t really contribute to the function

Question 5

IgM structure

Answer 5

Pentameric, always 5 antibodies in a circle

Question 6

IgA formation

Answer 6

Dimeric, can be free

Question 7

Protein that aids in the formation of polymeric Ig

Answer 7

J chain

Question 8

Ig chains on Naive B cells

Answer 8

IgM and IgD

Question 9

Memory B cells Ig make up

Answer 9

IgG, IgA, IgE

Question 10

Agglutination

Answer 10

Clumping antigen mediated by IgM pentamers, readily removed by amcrophages

Question 11

Neutralization of virus

Answer 11

Antibody blocks binding to virus receptor, can’t enter cells

Question 12

Inhibition of bacterial adhesion by antibody

Answer 12

Binds to bacterial binding sites, inhibits binding

Question 13

Opsonization

Answer 13

Aggregation of immunoglobulin on bacterial surface, causes the activation of macrophages via Fcgamma receptors

Question 14

Fcgamma receptors

Answer 14

Receptors on phagocytes that recognize IgG that mediates opsonization

Question 15

Antibody dependent cellular phagocytosis (ADCP)

Answer 15

IgG specific for tumor antigens, mediated by Fcgamma receptor

Question 16

Antibody-dependent cellular cytotoxicity (ADCC)

Answer 16

Natural killer (NK) cells express Fc-receptors for IgG, induce apoptosis

Question 17

Mast-cell activation via IgE cross-linking

Answer 17

Mast cells accumulate IgE, when bound to substrate they cross link and trigger degranulization, immunity to worms and allergic reactions

Question 18

Fcepsilon receptor

Answer 18

On mast cells for IgE

Question 19

Ag-specific receptor (BCR) Ig type

Answer 19

All isotypes

Question 20

Agglutination Ig type

Answer 20

IgM

Question 21

Neutralization Ig type

Answer 21

IgG and IgA

Question 22

Complement activation Ig type

Answer 22

IgM and IgG

Question 23

Opsonization isotype

Answer 23

IgG

Question 24

ADCP isotype

Answer 24

IgG

Question 25

ADCC isotype

Answer 25

IgG

Question 26

Mast cell activation isotype

Answer 26

IgE

Question 27

Isotype transported across the placenta

Answer 27

IgG

Question 28

Isotype found in breast milk

Answer 28

Dimeric IgA

Question 29

Secretory component protein

Answer 29

Interacts with dimeric IgA to mediated transcytosis

Question 30

Common mucosal immune system

Answer 30

When a mucosal plasma cell secretes dimeric IgA at a particular mucosal site, the cell proliferates and migrates to all mucosal sites

Question 31

Single chain Fv

Answer 31

The variable part of heavy and light chain linked by a polypeptide linker, can be used to deliver toxins to specific sites