Antibody ID

Question 1

Homozygous Cells Rule Out

Answer 1

Kidd, Duffy, MNS

Question 2

Panel Rule Out Process

Answer 2

Use panel cells negative at every phase to rule out specificities at the top of the panel sheet
Two strikes rules it out

Question 3

Panel Write-Up Steps

Answer 3

1. List the antibody specificities that could not be ruled out
2. List the antibodies that are not likely because they are very rare
3. List the antibodies that are not likely because of their phase of reactivity
4. What is the most likely antibody
5. List the selected cells that would need to be tested in order to rule out the remaining antibodies

Question 4

Antibody ID

Answer 4

Panel of 11 group O cells with known phenotype
Variation of agglutination strength
Multiple antibodies, dosage, anti-P

Question 5

Enzyme Treatment

Answer 5

Enzymes destroy some antigens: Fya, Fyb, Xga, M, N, S, s
Enzymes enhance some blood groups: Kidd, Rh, Lewis, P, Ii
Used when patient has multiple antibodies
Must observe reactivity of the same panel cells with both enzyme treted and untreated reactions and then compare results

Question 6

Antibody Adsorption

Answer 6

Removal of antibody from patient serum by mixing red cells with corresponding antigen
Used for separation of multiple antibodies and removal of auto-antibodies to detect allo-antibodies

Question 7

Antibody Elution

Answer 7

Removal of antibody from RBC membrane
Application includes positive DAT, HDFN, transfusion reaction, and autoimmune disease
ID antibodies coating red cells in each of the conditions