Antibodies

Question 1

The process, in which antibodies are specifically selected to target molecules (named antigens) is called what?

Answer 1

Immunolabelling

Question 2

In immunolabelling, once the ‘primary antibody’ is bound to the antigen, what happens next?

Answer 2

The secondary antibody is added, which labels the primary antibody (which is bound to the antigen) by binding to the primary antibody itself.

Question 3

During immunolabelling, what is the ‘secondary’ antibody marked with to highlight it’s position, and how is it viewed in vitro?

Answer 3

The secondary antibody is marked with a fluorescent light of a particular colour called a fluorophore, which is viewed with a fluorescent microscope

Question 4

What specific class of animals produce antibodies?

Answer 4

Vertebrates