Antibodies

Question 1

Heterophile Antibodies

Answer 1

EBV (Infectious Mono)

Question 2

Anti-Alveolar Basement Membrane Antibodies

Answer 2

Goodpasture Sx

Question 3

Anti-Centromere Antibodies

Answer 3

Limited Scleroderma (CREST Sx)

Question 4

Anti-Cyclic Citrullinated Peptide Antibody

Answer 4

RA

Question 5

Anti-Cardiolipin Antibodies (ACAs)

Def’n, Seen In (2)?

Answer 5

Type of APLA (anti-phospholipid antibody).
↑risk of blood clots and/or pregnancy loss.

SEEN IN:

• Antiphospholipid Sx
• SLE

Question 6

Anti-DNA Topoisomerase I (Anti-Scl 70)

Seen In?

Answer 6

SEEN IN:

- Diffuse Scleroderma

Question 7

Anti-Desmoglein Antibodies (IgG antibodies)

Seen In, Appearance on Immunofluorescence (IF)?

Answer 7

SEEN IN: Pemphigus Vulgaris

IF:
- Ab around epidermal cells in “netlike” / reticular pattern

(Desmoglein = component of desmosomes.
Desmosomes connect keratinocytes in stratum spinosum.)

Question 8

Anti-DNase B Antibodies

Def’n,↑Levels In (2)?

Answer 8

Antibodies to DNase B (a substance produced by Group A Strep).

↑IN:

• Acute Post-Strep Glomerulonephritis
• Rheumatic Fever

Question 9

Anti-Glomerular Basement Membrane Antibodies

Answer 9

Goodpasture Sx: necrotizing glomerulonephritis with linear IgG deposition on glomerular basement membrane.

Question 10

Anti-dsDNA Autoantibodies

Seen In?

Answer 10

SEEN IN:

- SLE ( -> Renal Disease )

Question 11

Anti-Glutamate Decarboxylase Autoantibodies

Seen In?

Answer 11

SEEN IN:

- Type I DM

Question 12

Anti-Gliadin / Anti-Transglutaminase / Anti-Endomysial IgA Autoantibodies

Seen In?

Answer 12

SEEN IN:

- Celiac Disease

Question 13

Anti-Histone Antibodies

Seen In?

Answer 13

SEEN IN:

- Drug-Induced SLE

Question 14

Anti-Hemidesmosome Antibodies (IgG antibodies)

Seen In, Appearance on IF?

Answer 14

SEEN IN:
- Bullous Pemphigoid

(Hemidesmosomes in epidermal basement memb = below epidermis)

IF:
- Linear pattern at epidermal-dermal junction

Question 15

Anti-Jo1 Autoantibodies

Seen In?

Answer 15

SEEN IN:

- Dermato- + Polymyositis

Question 16

Antimicrosomal Antithyroglobulin Autoantibodies

Def’n, Seen In?

Answer 16

Sign of Thyroid damage.

SEEN IN:
- Hashimoto’s Thyroiditis

Question 17

Anti-Mitochondrial Antibodies (AMA)

Answer 17

Primary Biliary Cirrhosis

Question 18

Anti-Nuclear Autoantibodies (ANA): Anti-SSA / SSB
(ie Anti-Ro / -La)

Seen In (3)?

Answer 18

SEEN IN:

• Sjorgen’s Sx
• SLE
• Libman-Sacks Endocarditis

Question 19

Anti-Neutrophil Cytoplasmic Antibodies (ANCA)

Def’n, Seen In?

Answer 19

Autoantibodies (primarily IgG) against antigens in the cytoplasm of Neutrophils.

SEEN IN:
- Vasculites

Question 20

C-ANCA (Cytoplasmic ANCA / PR3-ANCA)

Def’n, Seen In (2)?

Answer 20

ANCA with CYTOPLASMIC + diffusely GRANULAR staining pattern.

SEEN IN:

• RPGN
• Wegener Granulomatosis (Granulomatosis w polyangitis)

Question 21

P-ANCA (Perinuclear ANCA / MPO-ANCA)

Def’n, Seen In (4)?

Answer 21

ANCA with PERINUCLEAR staining pattern.

SEEN IN:

• RPGN
• Churg-Strauss Sx
• Microscopic Polyangitis
• Polyarteritis Nodosa

Question 22

Anti-Platelet Antibodies

IgG against platelet antigens Gp 2b/3a

Answer 22

Idiopathic Thrombocytopenic Purpura

Question 22

Anti-Mi2 Autoantibodies

Seen In?

Answer 22

SEEN IN:

- Dermato- + Polymyositis

Question 23

Anti-Smith Autoantibodies

Def’n, Seen In?

Answer 23

Antibodies against Smith antigen: part of snRNAs + snRNPs

SEEN IN:
- SLE

Question 24

Anti-Smooth Muscle Autoantibodies Seen In?

Answer 24

SEEN IN: | - Autoimmune Hepatitis

Question 25

Anti-SRP Autoantibodies Seen In?

Answer 25

SEEN IN: | - Dermato- + Polymyositis

Question 26

Antithyroglobulin Autoantibodies Seen In?

Answer 26

SEEN IN: | - Hashimoto Thyroiditis

Question 27

Anti-Topoisomerase Antibodies (ie Anti-Scl70)

Answer 27

Diffuse Systemic Scleroderma

Question 28

Anti-U1 RNP Autoantibodies Seen In?

Answer 28

SEEN IN: | - Mixed Connective Tis Dz

Question 29

Rheumatoid Factor Def'n, Mech (4 steps), Seen In (2)?

Answer 29

Anti-IgG antibody (IgM against IgG). MECH: Attacks IgG in synovial tis -> Infl -> Destruction of articular cartilage -> Joint Fibrosis + potential Ankylosis (if joint isn't moved). SEEN IN: - Rheumatoid Arthritis - Rheumatic Fever

Question 30

Autoantibodies against Ca Channels

Answer 30

Lambert-Eaton Myasthenic Sx

Question 31

Autoantibodies against Insulin Seen In?

Answer 31

SEEN IN: - Type 1 DM (often) * May be seen years before clinical dz presents *

Question 32

Autoantibodies to Ribonucleoprotein Antigens: SS-A (Ro), SS-B (La) Seen In?

Answer 32

SEEN IN: | - Sjogren's Sx

Question 33

Autoantibodies against Ach Receptor Seen In?

Answer 33

SEEN IN: | - Myasthenia Gravis

Question 34

Autoantibodies against TSH receptor Seen In?

Answer 34

SEEN IN: Graves Dz

Question 35

Monoclonal Antibody Spike Seen In (4)?

Answer 35

SEEN IN: - Monoclonal Gammopathy of Undetermined Significance - Multiple Myeloma (IgG or IgA) - Primary Amyloidosis - Waldenstrom Macroglobulinemia (IgM)