Animal Cell Culture Flashcards

1
Q

Animal cell culture

A

When cells from an intact organism are derived and isolated and maintained and grown in vitro aseptically containing a mixture of nutrients and growth factor.

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2
Q

Primary cell culture

A

A primary cell culture is when the cells are derived directly from the organism

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3
Q

What is the lifespan of the primary cell culture?

A

Limited

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4
Q

Why does the primary cell culture have a limited lifespan?

A

Because as they progressively divide the telomere shortens each time

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5
Q

Name two types of cell culture

A

Attachment culture and suspension culture

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6
Q

What is attachment culture?

A

When the cells need an attachment or anchorage to survive and grow

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7
Q

Give example of attachment culture

A

Kidney cells

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8
Q

What is suspension culture?

A

When the cells do not need any surface to grow

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9
Q

Give example of suspension culture

A

Lymphocytes

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10
Q

What is a cell line?

A

When a primary cell culture is subcultured

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11
Q

Two types of cell line.

A

Finite and infinite cell lines

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12
Q

What is finite cell line?

A

The cell line that eventually undergoes senescence

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13
Q

What is infinite cell line?

A

The cell line that can be subcultured indefinitely

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14
Q

Example of finite and infinite cell lines

A

Finite - mammalian cells
Infinite - cancer cells or transformed cells

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15
Q

What properties do finite cell line shows?

A

Contact inhibition, density limitation and anchorage dependence

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16
Q

Multilayer culture formed in?

A

Infine cell line

17
Q

First cell line derived from?

A

Mouse cells

18
Q

Lag phase

A

Period of zero growth
Cells get adapted
Undergoes trypsinization, secrete matrix aid for attachment

19
Q

Log or exponential growth phase

A

When the cells start doubling on the basis of their growth rate and seeding density

20
Q

Plateau or stationary phase

A

When there’s no more nutrients left and all the space has been occupied

21
Q

A typical animal cell culture media contains?

A

Amino acids, organic and inorganic acids, hormone, growth factor, vitamins, glucose and serum

22
Q

Two types of cell culture

A

Artificial and natural

23
Q

Natural media

A

Consist of biologically natural fluid

24
Q

Disadvantage of natural media

A

Poor reproducibility

25
Artificial media
Components are prepared artificially by adding particular solvents (nutrients)
26
Types of artificial media
Serum containing media Serum free media Chemically defined media Protein free media
27
Name the most common type of serum
Fetal bovine
28
Function of serum
Stimulates cell growth and cell activities Enhance cell attachment Provide transport proteins carrying hormones
29
Serum free media is used for?
To culture a specific type of cell line
30
Chemically defined media
Media contains ultra pure inorganic and organic, pure protein. It is contamination free
31
Protein free media used for?
Doesn't have proteins For superior cell growth and protein expression