Animal Cell Culture Flashcards
Animal cell culture
When cells from an intact organism are derived and isolated and maintained and grown in vitro aseptically containing a mixture of nutrients and growth factor.
Primary cell culture
A primary cell culture is when the cells are derived directly from the organism
What is the lifespan of the primary cell culture?
Limited
Why does the primary cell culture have a limited lifespan?
Because as they progressively divide the telomere shortens each time
Name two types of cell culture
Attachment culture and suspension culture
What is attachment culture?
When the cells need an attachment or anchorage to survive and grow
Give example of attachment culture
Kidney cells
What is suspension culture?
When the cells do not need any surface to grow
Give example of suspension culture
Lymphocytes
What is a cell line?
When a primary cell culture is subcultured
Two types of cell line.
Finite and infinite cell lines
What is finite cell line?
The cell line that eventually undergoes senescence
What is infinite cell line?
The cell line that can be subcultured indefinitely
Example of finite and infinite cell lines
Finite - mammalian cells
Infinite - cancer cells or transformed cells
What properties do finite cell line shows?
Contact inhibition, density limitation and anchorage dependence
