Amelogenesis - hard tissue development

Question 1

Enamel is a mineralised ? product. Dentine, cementum and bone are essentially mineralised ?product.

Answer 1

epithelial
connective tissue

Question 2

does enamel contain collagen?

Answer 2

no

Question 3

remember too much fluoride can interfere with amelogenesis

Question 4

remember

Question 5

Answer 5

ameloblasts

Question 6

Answer 6

odontoblasts

Question 7

what makes the ameloblasts produce enamel?

Answer 7

Presence of mineralised dentine

If dentine doesn’t mineralise enamel wont form

Question 8

amelogenesis

Epithelial cells get ? and their ?polarises to one side as the odontoblasts lay down predentine

Once dentine is formed the ??disintegrates

pre-ameloblasts become ameloblasts and form enamel

Ameloblasts retreat ? and ?

As the crystals get bigger the ?? is broken down and ? by ?

The ameloblasts change shape and get a point at the end called the ??

Once most of enamel is laid down the Tomes process is ? and becomes a folded cell membrane to increase ?? to ? matrix making the final enamel ?

Final ameloblast secretion -> ??

When the job is done the shrunken ameloblast becomes the ???

Answer 8

longer
nucleus

basal lamina

upwards
outwards

organic matrix
removed
ameloblasts

tomes process

lost
surface area
remove
aprismatic

enamel cuticle

reduced enamel epithelium

Question 9

The odontoblast processes that pierce the IEE become what?

Answer 9

enamel spindles

Question 10

the tomes process on ameloblasts

Gives ? enamel so u can ?it

The crystals ? off the end of the tomes process

Crystals ? depends on their ? from the tomes process and the ? of retreat of the ameloblasts

Regions formed without a tomes process are ?

what are the 2 aprismatic regions of enamel?

Answer 10

prismatic
etch

align

angulation
location
direction

aprismatic

first formed enamel
last formed enamel

Question 11

Note there is not pre-enamel -> it mineralises straight away

Question 12

overview amelogenesis

Question 13

Organic matrix proteins in enamel are epitactic agents that aid the formation of enamel

name 3

Answer 13

amelogenins
enamelins
tuftelin

Question 14

function of amelogenins

Answer 14

Used in root development

Clinically useful as theoretically can paint them onto root of teeth that have lost bone to produce more cementum, PDL and bone

Removed once enamel formed

Question 15

what are enamelins?

Answer 15

The matrix that remains after the rest is removed

Question 16

Development and maturation of inner enamel epithelium cells

Simple ? cell

Matures to ? cell with ?? and ? -> longest just before ? is produced

Initially no ?? so ? first 5um

Then get ?? and ? enamel

Get ? and ? membrane at the end so get ?? enamel

Answer 16

cuboidal

columnar
polar nucleus
elongates
enamel

tomes process
aprismatic

tomes process
prismatic

shorter
folded
smooth aprismatic

Question 17

Development and maturation of inner enamel epithelium cells

Question 18

fate of reduced enamel epithelium