Adult neurogenesis

Question 1

3H method

Answer 1

pulse-chasing
nucleoside analogue
incorporated into DNA
while neurons are dividing
shows neural development

Question 2

sequence of adult neurogenesis

Answer 2

1. radial glia like stem cells
2. progenitor cells
3. immature granule cells
4. new mature granule cells

Question 3

markers for immature cells

Answer 3

lenti and retro virus
have a preference for them

Question 4

chemotherapeutics in neurogenesis research

Answer 4

kill dividing cells
but: ALL dividing cells
many side effects

Question 5

exercise effect in rats

Answer 5

more new cells
more cell survival
more LTP
only if environment is challenging!

Question 6

age effect on neurogenesis explanations

Answer 6

metabolism?
inflammation?
age-regulated?

Question 7

neurogenesis in other areas

Answer 7

in hypothalamus and olfactory bulb
some in cortex after insults

Question 8

issues with neurogenesis research

Answer 8

requires good brain bank
multiple cases per age range
short time difference between death and autopsy

Question 9

neurogenesis marker

Answer 9

doublecortin
exclusively in developing neurons
associated with migration & microtubule

Question 10

Boldrini paper on age in different cell types

Answer 10

quiescent stem cells decrease with age
progenitor and immature are stable
angiogenesis and neuroplasticity decrease
granule, glia and dentate are stable