A2.2 Cell Structure Flashcards
Theory of spontaneaous generation
Belief that organisms could randomly appear from non-living matter
Cell theory
- smallest unit
- only arise from preexisting
all living things are made up of cells
Process of preparing wet mounts
- sharp scalpel: cut square out of onion
- tweezers: peel off thin inside layer
- transfer thin layer on glass slide
- pipette: add small drop of I2 = stain
- start cover slip at 90deg. = gently lower = avoid bubbles
- if bubbles= press w/pencil
Eyepiece garticule
Transparent ruler that is placed in eyyepiece lens to measure actual size
Light microspcope
- inexpensive
- magnifies up to 2000x
- specimens can be dead/alive
Resolution
The minimum distance between two points at which they can still be distinguished
Stage Micrometer
small, calibrated ruler mounted on stage microscope: precise distnace measurement
units of measurement
metre= 1x10^0
centimetre= 1x10^-3
millimetre= 1x10^-3
micrometre= 1x10^-6
nanometre = 10^9
Electron microscope
- expensive
- preperation of specimen is complex
- magnifies up to 500 000x
- specimen has to be dead
total magnification
ocular x objective
Cryogenic electron microscopy
- specimen is frozen
- image using computer enhancemnets (3D)
ex: virus & protein structures
Freeze Fracture
preparing sample for electron microscopy
* specimen rapidly frozen
* physically broken apart = internal structure
ex: cell membrane
Fluroscene
cells are stained with special dyes that bind to cellular components
=> UV shone
Immunofluoroscene
Antibodies combined w/dye added to specimen
=> bind to their target (UV)
Functions of Life
MR Sheng
Metabolism, Respiration, Sensitivity, Homeostasis, Excretion, Noutrition, Growth
Prokaryotes
Organisms that do not have proper nucleus enclosing their genetic material
Prokaryote contents
- nucleoid region
- ribosomes 70
- capsule
- plasmids
- pilli
- cell wall
- plasma membrane
*flagellum
Prokaryote Replication
- chromosome is replicated
- DNA copies move to opposite cell end
- cell elongates
- cell membrane grows inward = pinches off = two identical
- semi conservative replication
Eukarytotes
Organisms that contain a well defined nucleus and membrane bound cytoplasmic organelles
Eukaryote (animal) Content
- nucleolus
- nucleus
- vacuole
- Ribosome 80s
- cytoplams
- mitochondrion
- RER
- golgi.
- golgi- & vesicles
- SER
- cytoskeleton
Eukaryote (plant) Content
- same animal
+cell wall, choloroplast,
Eukaryote (fungal) Content
same animal
+Lysosomes, Peroxisome, centriole
All eukaryotes overlap
- nucleus
- vacuoles
- mitochondria
- plasma membrane
Only animal cells
- cillia
- Lysosomes