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Elsevier Mycology (RVSP) > 9. Dimorphic Fungi > Flashcards

9. Dimorphic Fungi Flashcards

1
Q

Primary infection of dimorphic fungi

A

Pulmonary

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2
Q

How are tissue specimens processed?

A

Minced

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3
Q

How are pleural fluid and CSF specimens processed?

A

Concentrated

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4
Q

How are mucus and pus specimens processed?

A

Mucolytic agent

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5
Q

Easiest and quickest method for microscopic examination for dimorphic fungi

A

KOH preparation (+ Calcofluor)

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6
Q

Stains used for in situ staining

A
  • H&E
  • PAS
  • GMS
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7
Q

Culture media for primary isolation of dimorphic fungi

A

SDA
SABHI
BHI

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8
Q

Incubation temp of media for primary isolation of dimorphic fungi

A

30°C

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9
Q

Media used for subculture for dimorphic fungi

A

PDA

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10
Q

MOT of dimophic fungi

A

Respiratory route

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11
Q

Immune response to dimorphic fungi

A

Cellular immune response

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12
Q

True/False. Dimorphic fungi are not strong antigens.

A

True

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13
Q

Explain dimorphic fungi antibody detection

A

Positive: exposure (not necessarily infection)

AIDS patients will be negative

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14
Q

What is detected in the expantigen test for dimorphic fungi?

A

Cell free extracts of the fungus

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15
Q

Used to react with known antisera in the exoantigen test for dimorphic fungi

A

Material extracted from mold phase

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16
Q

Causative agent of Gilchrist’s disease

A

Blastomyces dermatitidis

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17
Q

Disease caused by B. dermatitidis

A

Blastomycosis/Gilchrist’s disease

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18
Q

2 types Blastomycosis

A
  • chronic cutaneous blastomycosis

- systemic blastomycosis

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19
Q

Most common manifestations of systemic blastomycosis

A
  • bone lesions

- osteomyelitis

20
Q

After how many days will yeast forms of B. dermatitidis appear on enriched media?

A

After 10-15 days

21
Q

Associated with Valley fever

A

Coccidioides immitis

22
Q

The most virulent of all agents of human mycoses

A

Coccidioides immitis

23
Q

Disease caused by C. immitis

A

Primary pulmonary coccidiodomycosis

24
Q

After how many days will C. immitis appear on SDA/SABHI?

A

3-5 days

25
Q

Arthroconidia of C. immitis appear on SDA/SABHI after

A

7-10 days

26
Q

Has whote and cottony colonies (cobwebs)

A

C. immitis

27
Q

Has barrel-shaped arthroconidia

A

C. immitis

28
Q

Associated with disjuncture cells

A

C. immitis

29
Q

A soil saprobe with high nitrogen content

A

Histoplasma capsulatum

30
Q

Associated with Spelunker’s disease

A

H. capsulatum

31
Q

Disease caused bu H. capsulatum

A

Histoplasmosis

32
Q

Cell where H. capsulatum organisms can be found

A

Inside macrophages (histiocytes)

33
Q

After how many days will the yeast form of H. capsulatum appear?

A

After 10-15 days

34
Q

Has macroconidia that become tuberculate with age

A

H. capsulatum

35
Q

A soil saprobe of acid soil

A

Paracoccidioides brasiliensis

36
Q

Disease caused by P. brasiliensis

A

Paracoccidioidomycosis

37
Q

Mold colony of P. brasiliensis mature in

A

2-3 weeks

38
Q

Has a mariner wheel-shaped yeast form

A

P. brasiliensis

39
Q

Associated with Rose gardener disease

A

Sporothrix schenckii

40
Q

Disease caused by S. schenckii

A

Sporotrichosis

41
Q

Mold form of S. schenckii develops in

A

3-5 days

42
Q

Has hyphae frequently found as ropes

A

S. schenckii

43
Q

Has conidiophores that produce multiple conidia in flowerets arrangement

A

S. schenckii

44
Q

Has a yeast form that is cigar-shaped

A

S. schenckii

45
Q

Enumerate the thermal dimorphs

A
  • Blastomyces dermatitidis
  • Coccidioides immitis
  • Histoplasma capsulatum
  • Paracoccidioides brasiliensis
  • Sporothrix schenckii
  • Penicillium marneffei

Elsevier Mycology (RVSP) (13 decks)

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